Unique contributions of parvalbumin and cholinergic interneurons in organizing striatal networks during movement
Unique contributions of parvalbumin and cholinergic interneurons in organizing striatal networks during movement
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DOI：
10.1038/s41593-019-0341-3
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发表时间：
2019-04-01
影响因子：
25
通讯作者：
Han, Xue
中科院分区：
文献类型：
--
作者：
Gritton, Howard J.;Howe, William M.;Han, Xue
纹状体小白蛋白（PV）和胆碱能中间神经元（CHIs）通过协调传递运动输出的中棘细胞网络，在行为中发挥重要作用。然而，这些细胞数量少且分布分散，阻碍了对它们在行为过程中对中棘神经元（msn）网络活动的贡献的直接评估。在这里，我们基于单细胞钙成像技术的最新进展，结合光遗传学来测试pv和CHIs对小鼠自主运动时MSN活性和行为的影响。我们发现pv和CHIs对MSN的活性有独特的影响，并在支持运动中发挥解离作用。PV电池通过精炼负责运动执行的MSN网络的激活来促进运动。相比之下，chi在MSN网络中同步活动，以表明运动回合的结束。这些结果为支持运动的纹状体网络活动提供了新的见解。
Striatal parvalbumin (PV) and cholinergic interneurons (CHIs) are poised to play major roles in behavior by coordinating the networks of medium spiny cells that relay motor output. However, the small numbers and scattered distribution of these cells have hindered direct assessment of their contribution to activity in networks of medium spiny neurons (MSNs) during behavior. Here, we build on recent improvements in single-cell calcium imaging combined with optogenetics to test the capacity of PVs and CHIs to affect MSN activity and behavior in mice engaged in voluntary locomotion. We find that PVs and CHIs have unique effects on MSN activity and dissociable roles in supporting movement. PV cells facilitate movement by refining the activation of MSN networks responsible for movement execution. CHIs, in contrast, synchronize activity within MSN networks to signal the end of a movement bout. These results provide new insights into the striatal network activity that supports movement.