A new human genetic resource: a DNA bank established as part of the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC)
A new human genetic resource: a DNA bank established as part of the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC)
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DOI：
10.1038/sj.ejhg.5200502
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发表时间：
2000-09-01
影响因子：
5.2
通讯作者：
Golding, J
中科院分区：
文献类型：
--
作者：
Jones, RW;Ring, S;Golding, J
我们描述了一种独特的人类DNA资源，它构成了雅芳怀孕和儿童纵向研究(ALSPAC)的一部分，这是一项纵向队列研究，涉及生活在英格兰地理区域的14,000名儿童及其家庭。DNA库将为寻找基因多态和常见炉灶结局之间的联系提供支持。采集适合提取DNA的血液样本的机会必然有限，而且样本本身往往被以不同的方式处理，并有不同的存储历史。由于需要最大限度地提高产量，DNA提取方法的选择对银行的成功至关重要，我们已经研究了各种商业和内部DNA提取方法的适用性。已采取各种步骤尽量减少样品地址和鉴定中的错误，包括使用移液机器人在96孔阵列之间稀释和转移样品，以提供适合于聚合酶链式反应的等量。该机器人已经被编程为能够处理浓缩的粘性DNA溶液。
We describe a unique human DNA resource forming part of the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC), a longitudinal cohort study involving 14 000 children and their families living in a geographically defined area of England. The DNA bank will underpin the search for associations between genetic polymorphisms and common hearth outcomes. The opportunities to collect blood samples suitable for DNA extraction are necessarily limited, and the samples themselves have often been treated in different ways and have varied storage histories. With the need to maximise yields, the choice of DNA extraction method is critical to the success of the bank and we have investigated the suitability of various commercial and in-house methods of DNA extraction. Various steps have been taken to minimise errors in sample address and identification, including the use of a pipetting robot for dilution and transfer of samples between 96-well arrays to provide aliquots suitable for PCR. The robot has been programmed to cope with concentrated viscous DNA solutions.