Mammalian orthologues of a yeast regulator of nonsense transcript stability
Mammalian orthologues of a yeast regulator of nonsense transcript stability
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DOI：
10.1073/pnas.93.20.10928
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发表时间：
1996-10-01
影响因子：
11.1
通讯作者：
Dietz, HC
中科院分区：
文献类型：
--
作者：
Perlick, HA;Medghalchi, SM;Dietz, HC
到目前为止，所有已被研究的真核生物都具有检测和降解含有提前信号终止翻译的转录本的能力，这种无义介导的RNA衰退过程在酿酒酵母中得到了最全面的研究，其中至少需要三个反式作用因子(Upf1p到Upf3p)。我们已经克隆了编码人和小鼠Upf1p同源物的cDNA，称为rent1(无义转录调节因子)，Rent1是第一个发现的哺乳动物蛋白质，它包含Upf1p中所有可能的功能元件，包括锌指和NTPase结构域，以及解旋酶超家族I成员共同的所有基序，此外，还表达了嵌合蛋白包含rent1的中心区域，两侧是Upf1p的极端N和C末端，补充了酵母中Upf1p缺乏的表型，因此，尽管酵母和哺乳动物无义介导的RNA衰退之间存在明显的差异，这些数据表明这两条途径使用功能相关的机制。
All eukaryotes that have been studied to date possess the ability to detect and degrade transcripts that contain a premature signal for the termination of translation, This process of nonsense-mediated RNA decay has been most comprehensively studied in the yeast Saccharomyces cerevisiae where at least three trans-acting factors (Upf1p through Upf3p) are required, We have cloned cDNAs encoding human and murine homologues of Upf1p, termed rent1 (regulator of nonsense transcripts), Rent1 is the first identified mammalian protein that contains all of the putative functional elements in Upf1p including zinc finger-like and NTPase domains, as well as all motifs common to members of helicase superfamily I, Moreover, expression of a chimeric protein, containing the central region of rent1 flanked by the extreme N and C termini of Upf1p, complements the Upf1p-deficient phenotype in yeast, Thus, despite apparent differences between yeast and mammalian nonsense-mediated RNA decay, these data suggest that the two pathways use functionally related machinery.