Detection of clonal KPC-2-producing Klebsiella pneumoniae ST258 in Korea during nationwide surveillance in 2011.
Detection of clonal KPC-2-producing Klebsiella pneumoniae ST258 in Korea during nationwide surveillance in 2011.
复制标题
2011 年韩国全国监测期间检测到克隆性产 KPC-2 肺炎克雷伯菌 ST258。
DOI：
10.1099/jmm.0.059428-0
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发表时间：
2013
影响因子：
3
通讯作者：
Y. Lee
中科院分区：
文献类型：
--
作者：
J. Yoo;Hye Mee Kim;J. Yoo;Ji Woo Yang;H. Kim;G. Chung;Y. Lee
本研究分析了韩国产碳青霉烯酶（KPC-2）肺炎克雷伯菌分离株的特征和遗传相似性。最近的实验室监测发现韩国产碳青霉烯酶肠杆菌科增加。共筛选出6株产KPC-2的K.从277株肠杆菌科临床分离株中鉴定出肺炎链球菌。均为序列型（ST）258，脉型相同。它们对碳青霉烯类抗生素有较高的MIC，并具有多重耐药性。TEM-1型、SHV-11型和OXA型β-内酰胺酶均检出，未检出CTX-M型和质粒介导的AmpC酶（PABL）。接合实验失败，但使用来自6个分离株的含有blaKPC-2的质粒通过电穿孔转化大肠杆菌DH 5-α。每个转化体均携带一个约95 kb的blaKPC-2阳性质粒，该质粒在IncFII不相容性组中分型，并共携带TEM-1和OXA-9 β-内酰胺酶。他们有着相同的限制条件。本研究证实了克隆ST 258 KPC-2生产K的出现。在韩国的一些地区。
This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumoniae were identified from 277 Enterobacteriaceae clinical isolates. All were sequence type (ST) 258 and they had the same pulsotype. They had high MICs for carbapenems and multi-drug resistance. TEM-1, SHV-11 and OXA type β-lactamases were detected in all isolates, whereas CTX-M type β-lactamases and plasmid-mediated AmpC β-lactamase (PABL) were not present. A conjugation experiment failed, but blaKPC-2-harbouring plasmids from the six isolates were used to transform Escherichia coli DH5-α by electroporation. Each of the transformants harboured a blaKPC-2-positive approximately 95 kb plasmid, which was typed in the IncFII incompatibility group and co-harboured TEM-1 and OXA-9 β-lactamases. They shared the same restriction profile. This study confirms the emergence of clonal ST258 KPC-2-producing K. pneumoniae in some regions of Korea.