In silico integration of quantitative trait loci for seed yield and yield-related traits in Brassicanapus
In silico integration of quantitative trait loci for seed yield and yield-related traits in Brassicanapus
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DOI：
10.1007/s11032-013-0002-2
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发表时间：
2013
影响因子：
3.1
通讯作者：
Qingwen Zhou;D. Fu;A. Mason;Yongjun Zeng;Chao-Xian Zhao;Ying-Jin Huang
中科院分区：
文献类型：
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作者：
Qingwen Zhou;D. Fu;A. Mason;Yongjun Zeng;Chao-Xian Zhao;Ying-Jin Huang
数量性状基因座定位是分子标记辅助选择和图位基因克隆的基础。在过去的十年中，对甘蓝型油菜种子产量和产量相关性状进行了大量的QTL定位。已经被确认了。然而，由于研究之间缺乏共同的分子标记，因此还没有对这些结果进行整合，以比较来自不同作图群体的QTL。利用已报道的甘蓝型油菜和甘蓝型油菜基因组序列，在过去十年的油菜作图实验中，我们对与SY和产量相关的13个性状的1,960个QTL进行了电子整合。共有736个SY和产量相关的QTL被定位到B的A和C基因组的283个座位上。油菜籽。这些QTL在19B中分布不均。油菜染色体，最多位于A3染色体上，最少位于C6染色体上。我们的整合QTL图谱共检测到142个保守QTL和25个多功能基因座，主要涉及开花时间、株高、千粒重、成熟期和SY等性状。这些保守的QTL和多功能基因座可能是多效性或基因聚集性的结果。同时，通过与拟南芥基因组的比较定位，共确定了146个控制FT和其他产量相关性状的QTL基因。这些结果为B的检索提供了便利。为研究界提供NapusSY和产量相关的QTL，增加目标QTL连锁标记的密度，验证不同群体中QTL的存在，推进基因的精细定位。
Mapping quantitative trait loci (QTLs) is a foundation for molecular marker-assisted selection and map-based gene cloning. During the past decade, numerous QTLs for seed yield (SY) and yield-related traits inBrassicanapusL. have been identified. However, integration of these results in order to compare QTLs from different mapping populations has not been undertaken, due to the lack of common molecular markers between studies. Using previously reportedBrassica rapaandBrassica oleraceagenome sequences, we carried outin silicointegration of 1,960 QTLs associated with 13 SY and yield-related traits from 15B.napusmapping experiments over the last decade. A total of 736 SY and yield-related QTLs were mapped onto 283 loci in the A and C genomes ofB. napus. These QTLs were unevenly distributed across the 19B. napuschromosomes, with the most on chromosome A3 and the least on chromosome C6. Our integrated QTL map identified 142 loci where the conserved QTLs were detected and 25 multifunctional loci, mostly for the traits of flowering time (FT), plant height, 1,000-seed weight, maturity time and SY. These conserved QTLs and multifunctional loci may result from pleiotropism or clustered genes. At the same time, a total of 146 genes underlying the QTLs for FT and other yield-related traits were identified by comparative mapping with theArabidopsisgenome. These results facilitate the retrieval ofB. napusSY and yield-related QTLs for research communities, increase the density of targeted QTL-linked markers, validate the existence of QTLs across different populations, and advance the fine mapping of genes.