Construction, Expression, and Characterization of rSEA-EGF and In Vitro Evaluation of its Antitumor Activity Against Nasopharyngeal Cancer.
Construction, Expression, and Characterization of rSEA-EGF and In Vitro Evaluation of its Antitumor Activity Against Nasopharyngeal Cancer.
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rSEA-EGF的构建、表达和表征及其抗鼻咽癌活性的体外评价
DOI：
10.1177/1533033818762910
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发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Tao A
中科院分区：
文献类型：
--
作者：
Liu X;Zeng L;Zhao Z;Xie Y;Wang S;Zhang J;He Y;Zou Z;Zhang J;Tao A
葡萄球菌肠毒素 A 是众所周知的超级抗原，可用于癌症免疫治疗。本研究将重组葡萄球菌肠毒素A与表皮生长因子进行基因缀合，产生在大肠杆菌中表达的嵌合蛋白重组葡萄球菌肠毒素A-表皮生长因子。采用Strep-Tactin亲和层析和内毒素去除树脂纯化重组葡萄球菌肠毒素A-表皮生长因子蛋白，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和液相色谱-串联质谱分析进行鉴定。此外，体外实验表明，纯化的重组葡萄球菌肠毒素A-表皮生长因子能够成功地与人鼻咽癌细胞系CNE2结合，显着促进人外周血单个核细胞的增殖，并增强多种具有广泛抗肿瘤活性的细胞因子的分泌，如干扰素-γ、肿瘤坏死因子-α和白细胞介素-2。重要的是，当与外周血单核细胞共培养时，重组葡萄球菌肠毒素A-表皮生长因子显着抑制CNE2细胞的增殖并促进CNE2细胞的凋亡。最后，重组葡萄球菌肠毒素A-表皮生长因子的结合和重组葡萄球菌肠毒素A-表皮生长因子激活的外周血单核细胞的毒性均被证明具有特异性，并且仅对高表皮生长因子受体表达细胞系有效。总之，我们的工作表明重组葡萄球菌肠毒素 A-表皮生长因子可作为一种有前途的新型免疫治疗剂。需要更多的体内和体外研究来验证其抗肿瘤效力，并研究癌症免疫治疗的潜在机制。
Staphylococcal enterotoxin A is well known as a superantigen and able to be used for cancer immunotherapy. In this study, recombinant Staphylococcal enterotoxin A was genetically conjugated to epidermal growth factor to produce a chimeric protein recombinant Staphylococcal enterotoxin A–epidermal growth factor expressed in Escherichia coli. The recombinant Staphylococcal enterotoxin A–epidermal growth factor protein was purified using Strep-Tactin affinity chromatography and Endotoxin Removal Resin and identified by sodium dodecyl sulfate-polyacrylamide gel electropheresis and liquid chromatography-tandem mass spectrometry analysis. Furthermore, in vitro experiments showed purified recombinant Staphylococcal enterotoxin A–epidermal growth factor could successfully bind to the human nasopharyngeal carcinoma cell line CNE2, significantly promote the proliferation of human peripheral blood mononuclear cells, and enhance the secretion of several cytokines that have broad antitumor activities, such as interferon-γ, tumor necrosis factor-α, and interleukin-2 . Importantly, recombinant Staphylococcal enterotoxin A–epidermal growth factor significantly inhibited proliferation of CNE2 cells and promoted apoptosis in CNE2 cells when cocultured with peripheral blood mononuclear cells. Finally, both the binding of recombinant Staphylococcal enterotoxin A–epidermal growth factor and the toxicity of recombinant Staphylococcal enterotoxin A–epidermal growth factor-activated peripheral blood mononuclear cells were demonstrated as specific and only effective on high epidermal growth factor receptor-expressing cell lines. In all, our work suggests that recombinant Staphylococcal enterotoxin A–epidermal growth factor serves as a promising novel immunotherapeutic agent. More in vivo and in vitro studies are needed to verify its antitumor potency, as well as investigate the underlying mechanisms in cancer immunotherapy.
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影响因子：
168.9
作者：
Disis, Mary L.;Bernhard, Helga;Jaffee, Elizabeth M.
通讯作者：
Jaffee, Elizabeth M.
人类TGFALPHA衍生的肽TGFALPHAL3与超抗原融合，用于对EGFR表达肿瘤的免疫疗法。
影响因子：
3.5
作者：
Xu Q;Zhang X;Yue J;Liu C;Cao C;Zhong H;Ma Q
通讯作者：
Ma Q
影响因子：
--
作者：
Han, Conghui;Hao, Lin;Wu, Yongping
通讯作者：
Wu, Yongping
DOI：
10.1073/pnas.1617941114
发表时间：
2017-05-23
影响因子：
11.1
作者：
Ramagopal, Udupi A.;Liu, Weifeng;Almo, Steven C.
通讯作者：
Almo, Steven C.
影响因子：
5.6
作者：
Sundstedt, Anette;Celander, Mona;Hedlund, Gunnar
通讯作者：
Hedlund, Gunnar