Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types.
Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types.
复制标题
脂肪组织基质细胞对浅表型和浸润型膀胱尿路上皮癌凋亡、生长和侵袭的差异影响。
DOI：
10.1111/iju.13086
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Toda S.
中科院分区：
文献类型：
--
作者：
Kawasaki-Nanri M;Aoki S;Uchihashi K;Yamamoto M;Udo K;Nishijima-Matsunobu A;Kakihara N;Noguchi M;Uozumi J;Toda S.
目的探讨脂肪组织基质细胞与膀胱癌细胞之间的相互作用。方法将浅表性（RT 4）和浸润性（EJ）尿路上皮癌细胞在脂肪组织基质细胞包埋或非包埋的胶原凝胶上培养。采用免疫组化、western blot和真实的实时逆转录聚合酶链反应（RT-PCR）对细胞进行分析。相反，脂肪组织基质细胞促进EJ的生长，但不影响凋亡。脂肪组织基质细胞轻微促进RT 4和EJ中丝裂原活化蛋白激酶级联的表达。脂肪组织基质细胞仅在RT 4中促进分子靶向剂人表皮生长因子受体-2的展示。反过来，RT 4和EJ增强α-平滑肌肌动蛋白（肌成纤维细胞标志物）和S-100蛋白结论：（1）脂肪组织基质细胞可能抑制浅表型癌的进展，但可能促进浸润型癌的进展;（ii）脂肪组织基质细胞活化的丝裂原活化蛋白激酶途径可能在两种类型的膀胱癌中发挥不同的作用;（iii）人表皮生长因子受体-2可能代表脂肪组织基质细胞-癌症相互作用下浅表型的关键治疗剂;和（iv）浅表性膀胱癌可能促进脂肪组织基质细胞的肌成纤维细胞分化为癌症相关表型，而浸润性膀胱癌可能促进其脂肪细胞分化。
ObjectivesTo clarify the interaction between adipose tissue stromal cells and bladder cancer cells.MethodsSuperficial (RT4) and invasive (EJ) urothelial carcinoma cells were cultured on adipose tissue stromal cell‐embedded or non‐embedded collagen gel. Cells were analyzed by immunohistochemistry, western blot and real‐time reverse transcription polymerase chain reaction.ResultsAdipose tissue stromal cells inhibited growth of RT4, while they promoted the apoptosis. In contrast, adipose tissue stromal cells promoted growth of EJ, but they did not affect the apoptosis. Adipose tissue stromal cells slightly promoted expression of mitogen‐activated protein kinase cascade in RT4 and EJ. Adipose tissue stromal cells promoted display of the molecular‐targeted agent human epidermal growth factor receptor‐2 in only RT4. In turn, RT4 and EJ enhanced α‐smooth muscle actin (myofibroblast marker) and S‐100 protein (adipocyte marker) expression of adipose tissue stromal cells, respectively.ConclusionsThese findings suggest that: (i) adipose tissue stromal cells might suppress the progression of superficial‐type cancer, whereas they might promote that of invasive type; (ii) adipose tissue stromal cell‐activated mitogen‐activated protein kinase pathway might play differential roles in both types of bladder cancer; (iii) human epidermal growth factor receptor‐2 could represent a critical therapeutic agent for the superficial type under adipose tissue stromal cells–cancer interaction; and (iv) superficial bladder cancer might promote myofibroblast differentiation of adipose tissue stromal cells as a cancer‐associate phenotype, whereas invasive bladder cancer might promote their adipocyte differentiation.