Adenosine receptors in rat testes: labeling with 3H-cyclohexyladenosine.
Adenosine receptors in rat testes: labeling with 3H-cyclohexyladenosine.
复制标题
大鼠睾丸中的腺苷受体：用 3H-环己基腺苷标记。
DOI：
10.1016/0024-3205(81)90054-0
复制
发表时间：
1981
期刊：
影响因子：
6.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Murphy,KM;Snyder,SH
我们用~ 3 H-N-6-环己基腺苷（~ 3 H-CHA）标记大鼠组织中的腺苷受体，并评价了这些结合位点在全身的器官分布和药物特异性。可检测的受体浓度仅限于大脑和睾丸。脑显示受体水平为9 pmole/g组织湿重，而睾丸显示2 pmole/g，检查的所有其他组织显示低于可检测的3 H-CHA特异性结合。这两种组织中的受体似乎具有相同的药理学特征，两者均具有A1特异性。在处死前14天切除垂体的大鼠睾丸中，未观察到3 H-CHA结合位点总数的变化。这可能排除了大鼠睾丸中作为腺苷受体来源的间质细胞。
We have labeled adenosine receptors in rat tissues with3HN6cyclohexyladenosine (3HCHA) and evaluated the organ distribution and drug specificity of these binding sites throughout the body. Detectable receptor concentrations are restricted to the brain and testes. Brain displays receptor levels of 9 pmoles/g wet weight of tissue while the testes display 2 pmoles/g, with all other tissues examined showing less than detectable specific binding of3HCHA. The receptors in these two tissues appear to possess identical pharmacologic profiles with an A1specificity for both. No change in the total number of3HCHA binding sites is seen in testes of rats which had been hypophysectomized 14 days prior to sacrifice. This may exclude the Leydig cells as a source of adenosine receptors in the rat testes.