In Vivo Monitoring of Hydrogen Polysulfide via a NIR-Excitable Reversible Fluorescent Probe Based on Upconversion Luminescence Resonance Energy Transfer.
In Vivo Monitoring of Hydrogen Polysulfide via a NIR-Excitable Reversible Fluorescent Probe Based on Upconversion Luminescence Resonance Energy Transfer.
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DOI：
10.1021/acs.analchem.2c01650
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发表时间：
2022-06
影响因子：
7.4
通讯作者：
Qiang Duan;Yifan He;Wenqiang Bi;Tao Liang;Zhihong Liu;Zhen Li
中科院分区：
文献类型：
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作者：
Qiang Duan;Yifan He;Wenqiang Bi;Tao Liang;Zhihong Liu;Zhen Li
多硫化氢（H2Sn）是由硫化氢（H2S）衍生而来的，被认为是生理和病理过程中代替H2S的信号分子。通过NIR可激发荧光探针可逆检测H2Sn是了解其功能的有效手段，但具有相当大的挑战性。本文中，我们报道了一种基于发光共振能量转移原理的近红外可激发的比率纳米探针，该探针以上转换纳米粒子为能量供体，以有机分子SiR1为能量受体和H2Sn的可逆识别单元，用于可逆检测H2Sn。所制备的纳米探针对H2Sn的可逆检测具有高选择性和快速响应，可用于监测活细胞中内源性H2Sn的形成和消耗。由于近红外激发的自发荧光减弱，因此成功地应用于跟踪小鼠生理和病理过程中H2Sn浓度的波动，包括炎症和肝损伤。
Hydrogen polysulfide (H2Sn), derived from hydrogen sulfide (H2S), has attracted increasing attention, which is suggested to be the actual signal molecule instead of H2S in physiological and pathological processes. Reversible detection of H2Sn through a NIR-excitable fluorescence probe is an effective means to understand its functions but is quite challenging. Herein, we reported a NIR-excitable ratiometric nanoprobe for the reversible detection of H2Sn based on luminescence resonance energy transfer principle with upconversion nanoparticles as the energy donor and an organic molecule, SiR1, as the energy acceptor and reversible recognition unit of H2Sn. The as-prepared nanoprobe exhibited high selectivity and fast response for the reversible detection of H2Sn, which can monitor the formation and consumption of endogenous H2Sn in living cells. Because of the reduced autofluorescence by NIR excitation, it was successfully applied for tracking the fluctuation of H2Sn concentration of mice in physiological and pathological processes including inflammation and liver injury.