MECHANISM OF EFFECT OF NICOTINE ON RENAL WATER EXCRETION
MECHANISM OF EFFECT OF NICOTINE ON RENAL WATER EXCRETION
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DOI：
10.1152/ajplegacy.1974.227.5.1216
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发表时间：
1974-01-01
影响因子：
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通讯作者：
SCHRIER, RW
中科院分区：
文献类型：
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作者：
CADNAPAPHORNCHAI, P;BOYKIN, JL;SCHRIER, RW
方法在16只体重20-30 kg的杂种犬中进行了33次实验。研究前18小时禁食，但允许动物自由饮水。在研究的早晨，用静脉内戊巴比妥（30 μ g/kg）麻醉动物，插管并用哈佛呼吸器通气。在整个实验过程中，通过间断静脉注射小剂量戊巴比妥维持轻度麻醉。麻醉诱导后，所有动物肌内注射5 mg醋酸脱氧皮质酮油。在一些动物中，通过颊侧入路进行急性垂体切除术，之后动物肌内和静脉内接受0.8 mg地塞米松。在所有动物中，通过导管以20 ml/min的速度在前腿静脉中输注2.5 × 7葡萄糖和水的溶液50 min，在此期间进行以下手术。通过双侧腹膜后侧切口将聚乙烯导管放置在输尿管和肾静脉中。在第I组研究（见下文）中，通过剥离和切断肾神经，然后将100%酒精应用于肾蒂，在一些犬的一个肾脏中进行去神经支配。在将尼古丁注射到颈动脉中的研究中，将23号针头放置在左颈动脉中，并通过以0.5 ml/min的速度连续输注2.5%葡萄糖和水来保持通畅。
METHODSThirty-three experiments were performed in 16 mongrel dogs of either sex weighing 20-30 kg. Food was withheld 18 h before study but the animals were allowed free access to water. On the morning of study the animals were anesthetized with intravenous pentobarbital(30 “g/kg), intubated, and ventilated with a Harvard respirator. Light anesthesia was maintained throughout the experiment by the intermittent intravenous administration of small doses of pentobarbital. After induction of anesthesia all animals received 5 mg of deoxycorticosterone acetate in oil intramuscularly. In some animals acute hypophysectomy was performed through a buccal approach, after which the animals received 0.8 mg of dexamethasone both intramuscularly and intravenously. In all animals a solution of 2.5 7; glucose and water was infused through a catheter in a foreleg vein at 20 ml/min for 50 min, during which time the following surgery was performed. Polyethylene catheters were placed in both ureters and renal veins through bilateral retroperitoneal flank incisions. In the group I studies (see below) denervation was performed in one kidney of some dogs by stripping and severing the renal nerves and then applying 100% alcohol to the renal pedicle. In studies in which nicotine was injected into the carotid artery, a 23-gauge needle was placed in the left carotid artery and kept patent by a continuous infusion of 2.5% glucose and water at 0.5 ml/min. In 13 animals an ad-