HLA-DR α CHAIN EXPRESSION IN HUMAN THYROID CELLS
HLA-DR α CHAIN EXPRESSION IN HUMAN THYROID CELLS
复制标题
人类甲状腺细胞中 HLA-DR α 链的表达
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
T. Davies
中科院分区：
文献类型：
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作者：
L. Piccinini;B. Schachter;T. Davies
Using a cDNA probe encoding the human major histocompatibility Class II antigen HLA-DR a chain, we have detected a single DR a chain-specific transcript in total cellular RNA prepared from human thyroid tissue, the hybridizable RNA in thyroid samples was indistinguishable in size from the DR mRNA in the Raji human B lymphoblastoid cell line. Of the thyroid glands examined, samples from patients with autoimmune thyroid disease consistently demonstrated the highest DR a chain transcript levels, with a mean ± sem OF 62±13% of levels found in DR-positive Raji cells. Cytoplasmic dot blot analyses of 5-day thyroid cell cultures depleted of lymphocytes and monocytes indicated that normal thyrocytes contain readily-detectable levels of DR a chain-mRNA. These transcript levels varied from individual to individual, with a mean of 16±9% relative to Raji cell control values and were shown to correlate after lectin or gamma interferon stimulation with enhanced numbers of immunoreactive DR antigen-positive cells. Such ...
