Selective fishing and analysis of xanthine oxidase binders from two Fabaceae species by coupling enzyme functionalized core-shell magnetic nanoparticles with HPLC-MS
Selective fishing and analysis of xanthine oxidase binders from two Fabaceae species by coupling enzyme functionalized core-shell magnetic nanoparticles with HPLC-MS
复制标题
通过酶功能化核壳磁性纳米粒子与 HPLC-MS 耦合对两种豆科物种的黄嘌呤氧化酶结合物进行选择性捕获和分析
DOI：
10.1016/j.jchromb.2013.11.053
复制
发表时间：
2014-01-15
影响因子：
3
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Liu, Liangliang;Shi, Shuyun;Chen, Xiaoqing
Xanthine oxidase (XOD) immobilized core-shell magnetic silica (Fe3O4@SiO2-XOD) nanoparticles coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) was developed to fish out and analyze XOD binders from two Fabaceae species, Puerariae lobata flower and Glycyrrhiza uralensis root. The prepared Fe3O4@SiO2-XOD nanoparticles exhibited good specificity for XOD binders, better dispersion in aqueous solution and reusability than those of Fe3O4-XOD nanoparticles. The amount of XOD immobilized onto Fe3O4@SiO2 nanoparticles was 339.9 mu g/mg and the activity of Fe3O4@SiO2-XOD nanoparticles remained 95% after ten times usage. The optimum conditions of selective fishing were optimized, and finally incubating pH was set at 7, incubating temperature at 25 degrees C and adsorption time at 30 min. Twelve XOD binders were successfully identified from ethyl acetate extract of P. lobata flower and G. uralensis root. The developed method provides a rapid, purposeful and effective way to identify active compounds from natural complex mixtures. (C) 2013 Elsevier B.V. All rights reserved.
