Exosomal miR-214-3p as a potential novel biomarker for rhabdoid tumor of the kidney
Exosomal miR-214-3p as a potential novel biomarker for rhabdoid tumor of the kidney
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DOI：
10.1007/s00383-021-04989-w
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发表时间：
2021-09
影响因子：
1.8
通讯作者：
Bin Yamaoka;Eri Nagasaki-Maeoka;Shota Uekusa;Ayano Hidaka;Kohei Hijikata;Reona Kato;K. Fujiwara;T. Koshinaga;S. Uehara
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文献类型：
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作者：
Bin Yamaoka;Eri Nagasaki-Maeoka;Shota Uekusa;Ayano Hidaka;Kohei Hijikata;Reona Kato;K. Fujiwara;T. Koshinaga;S. Uehara
目的肾横纹肌样肿瘤(RTK)是一种少见的、高度侵袭性的儿童肾脏肿瘤。目前还没有检测RTK的特异性生物标志物，与包括神经母细胞瘤(NB)在内的其他儿童腹部肿瘤的初步鉴别诊断也很困难。外体miRNAs是一种可以在生物液中检测到的新型癌症生物标记物。方法从人RTK来源的细胞系、人胚胎肾细胞系和人NB来源的细胞系的条件培养液中收集RTK来源的外体miRNAs。采用miRNA测序技术(miRNA-Seq)检测RTK特异性外体miRNAs。用定量逆转录聚合酶链式反应(qRT-PCR)检测肿瘤细胞株和RTK移植小鼠血清条件培养液中外体miR-214-3p的表达。QRT-PCR检测RTK来源的细胞系外体miR-214-3p的表达水平显著高于人胚胎肾细胞系和NB来源的细胞系。此外，RTK移植小鼠血清外体miR-214-3p的表达水平明显高于对照组。结论外体miR-214-3p有可能成为RTK新的生物标志物。
PurposeRhabdoid tumor of the kidney (RTK) is a rare, highly aggressive pediatric renal tumor. No specific biomarkers are available for detection of RTK, and the initial differential diagnosis from other pediatric abdominal tumors, including neuroblastoma (NB), is difficult. Exosomal miRNAs are novel cancer biomarkers that can be detected in biological fluids. We explored candidate RTK-specific exosomal miRNAs as novel biomarkers of RTK.MethodsExosomal miRNAs were collected from conditioned media of human RTK-derived cell lines, a human embryonic renal cell line, and human NB-derived cell lines. miRNA sequencing (miRNA-Seq) was performed to detect candidate RTK-specific exosomal miRNAs. The exosomal miRNA expression in conditioned media of tumor cell lines and serum from RTK xenograft-bearing mice was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).ResultsThe expression of exosomal miR-214-3p detected by miRNA-Seq was highest in RTK-derived cell lines. Exosomal miR-214-3p expression level determined by qRT-PCR was significantly higher in RTK-derived cell lines than in the human embryonic renal cell line or NB-derived cell lines. Furthermore, the serum exosomal miR-214-3p expression level was significantly higher in RTK xenograft mice than controls.ConclusionOur data indicated that exosomal miR-214-3p has potential as a novel biomarker of RTK.