DNA hybridization electrochemical biosensor using a functionalized polythiophene.
DNA hybridization electrochemical biosensor using a functionalized polythiophene.
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DOI：
10.1016/j.talanta.2009.03.049
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发表时间：
2009-07
期刊：
影响因子：
6.1
通讯作者：
A. Uygun
中科院分区：
文献类型：
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作者：
A. Uygun
通过电化学聚合噻吩-3-羧酸4-羟基苯酯，制备了一种简单、无标记的DNA传感器。采用电化学方法在玻碳电极上合成了聚噻吩-3-羧酸4-羟基苯酯（PHPT），并用循环伏安法、傅里叶变换红外光谱（FTIR）和原子力显微镜（AFM）对其进行了表征。将ODN探针物理吸附到PHPT膜上，并与互补ODN片段杂交进行测试。生物识别可以通过与单链和双链状态寡核苷酸的电化学信号（循环伏安图）进行比较来监测。双链态寡核苷酸的氧化电流低于单链态寡核苷酸的氧化电流，这与聚合物结构刚度增加导致PHPT电活性降低相对应。用原子力显微镜观察了ODN探针在ITO电极上的物理吸附及其杂交。该传感器的灵敏度为0.02μA/nmol，检测限为1.49nmol，具有良好的选择性。
A simple and label-free electrochemical sensor for recognition of the DNA sensor event was prepared by electrochemical polymerization of 4-hydroxyphenyl thiophene-3-carboxylate. Poly(4-hydroxyphenyl thiophene-3-carboxylate) (PHPT) was synthesized electrochemically onto glassy carbon electrode and characterized by cyclic voltammetry, FTIR and AFM measurements. An ODN-probe was physisorbed onto PHPT film and tested on hybridization with complementary ODN segments. A biological recognition can be monitored by comparison with electrochemical signal (cyclic voltammogram) of single and double strand state oligonucleotide. The oxidation current of double strand state oligonucleotide is lower than that of single strand, that is corresponding to the decrease of electroactivity of PHPT with the increase of stiffness of polymer structure. Physisorbed ODN-probe and its hybridization were observed morphologically onto ITO electrodes using AFM. The sensitivity of the electrochemical sensor is 0.02μA/nmol, detection limit is 1.49nmol and it has good selectivity.