The role of smooth muscle cells in the fibrogenesis of arteriosclerosis.
The role of smooth muscle cells in the fibrogenesis of arteriosclerosis.
复制标题
DOI：
--
复制
发表时间：
1960-10
期刊：
影响因子：
--
通讯作者：
Haust;More Rh;Movat Hz
中科院分区：
文献类型：
--
作者：
Haust;More Rh;Movat Hz
将所有组织固定在改良的福尔马林-升华-乙酸（FSA）溶液或Lillie's缓冲的10%福尔马林中。在每种固定液的最佳固定时间之后，如别处所述，通过使用乙醇和甲苯的常规方法或通过四氢呋喃进行脱水、澄清、渗透和包埋的过程。厚度不超过3埃的切片用红明矾-荧光桃红-蓝红、Masson三色、磷钨酸-苏木因或磷钨酸苏木精、阿新蓝-PAS-苏木精-橙G、Heidenhain 'sazan、五色染料II、间苯二酚品红-nudear坚牢红-间胺黄和用于网状纤维的Bielschowsky-Maresch银浸渍-核坚牢红-间胺黄染色。
removed at All the tissues were fixed in a modified formol-sublimate-acetic (FSA) solutiont or Lillie's buffered iO per cent formalin.' Following the fixation time optimal for each fixative solution, the processes of dehydration, clearing, infiltration and embedding by either the conventional method using alcohol and toluol, or by means of tetrahydrofuran, were carried out as outlined elsewhere.' Sections not exceeding 3A in thickness were stained with hemalum-phloxine-saffron, Masson's trichrome, phosphotungstic-acid-hematein or phosphotungstic acid hematoxylin, Alcian blue-PAS-hematoxylin-orange G, Heidenhain's azan, pentachrome II, resorcin fuchsin-nudear fast red-metanil yellow, and Bielschowsky-Maresch silver impregnation for reticular fibers-nuclear fast red-metanil yellow.