Crystallographic analysis of RsmA, a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus.
Crystallographic analysis of RsmA, a ribosomal RNA small subunit methyltransferase A from Staphylococcus aureus.
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RsmA（金黄色葡萄球菌的一种核糖体 RNA 小亚基甲基转移酶 A）的晶体分析。
DOI：
10.1107/s2053230x15011279
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Li Xu
中科院分区：
文献类型：
--
作者：
Liu Yang;Zhu Yuwei;Teng Maikun;Li Xu
RSMA是金黄色葡萄球菌核糖体RNA小亚基甲基转移酶，催化16S rRNA中腺嘌呤的N6甲基化。本研究对金黄色葡萄球菌RSMA进行了克隆、表达、纯化和结晶。该晶体属于C2空间群，晶胞参数a=84.38，b=157.76，c=96.50 ä，β=95.04°。收集的X射线衍射数据的分辨率为3.2 ？自旋函数和马修斯系数表明在不对称单元中存在两个分子。
RsmA, a ribosomal RNA small subunit methyltransferase from Staphylococcus aureus, catalyzes the N6 methylation of adenine in 16S rRNA. In this study, RsmA from Staphylococcus aureus was cloned, expressed, purified and crystallized. The crystal belonged to space group C2, with unit-cell parameters a = 84.38, b = 157.76, c = 96.50 Å, β = 95.04°. X-ray diffraction data were collected to a resolution of 3.2 Å. The self-rotation function and the Matthews coefficient suggested the presence of two molecules in the asymmetric unit.