Predictions of novel two-photon absorption bands in fluorescent proteins
Predictions of novel two-photon absorption bands in fluorescent proteins
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DOI：
10.1021/jp075545v
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发表时间：
2007-12-20
影响因子：
3.3
通讯作者：
Luo, Yi
中科院分区：
文献类型：
--
作者：
Nifosi, Riccardo;Luo, Yi
利用时间依赖的密度泛函理论，我们计算了一些固有荧光蛋白模型发色团中最低相关激发的双光子截面。在不同的发色团中，第一、单光子主动跃迁的双光子强度是不同的，这与实验结果一致。有趣的是，正如少态模型分析所揭示的那样，在500-700 nm区域发现了由近共振增强引起的具有大双光子截面的额外跃迁。荧光蛋白在该光谱区域的多光子激发可以导致生物成像的相关应用。
By means of time-dependent density functional theory, we calculate the two-photon cross-sections for the lowest relevant excitations in some model chromophores of intrinsically fluorescent proteins. The two-photon strength of the first, one-photon active transition varies among the various chromophores, in line with experimental findings. Interestingly, additional transitions with large two-photon cross-sections are found in the 500-700 nm region arising from near-resonant enhancement, as revealed by few-state model analysis. Multiphoton excitation of fluorescent proteins in this spectral region can lead to relevant application for bioimaging.