Antibody response of children with enteropathogenic Escherichia coli infection to the bundle-forming pilus and locus of enterocyte effacement-encoded virulence determinants
Antibody response of children with enteropathogenic Escherichia coli infection to the bundle-forming pilus and locus of enterocyte effacement-encoded virulence determinants
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DOI：
10.1086/314549
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发表时间：
1999-01-01
影响因子：
6.4
通讯作者：
Girón, JA
中科院分区：
文献类型：
--
作者：
Martinez, MB;Taddei, CR;Girón, JA
肠致病性大肠杆菌（EPEC）表达一种质粒编码的IV型菌毛，称为菌毛形成菌毛，它与培养的上皮细胞上细菌小菌落的形成有关。肠上皮细胞刷状缘膜的细菌附着和消失归因于被称为内膜素的表面外膜蛋白粘附素和EPEC分泌的蛋白EspA、EspB和EspD。除内膜素外，在幼儿中的自然EPEC感染期间，尚未证实体内产生或针对这些毒力决定因素的抗体应答。对巴西自然感染EPEC的儿童进行了抗BfpA、intimin、EspA和EspB的抗体应答调查。因此，肠上皮细胞附着编码产物的形成菌毛和位点在天然EPEC感染期间在体内产生，并引发针对异源EPEC毒力决定簇的免疫应答。这些发现对EPEC感染的免疫预防具有重要意义。
Enteropathogenic Escherichia coli (EPEC) express a plasmid-encoded type IV pilus termed bundle-forming pilus, which is associated with the formation of bacterial microcolonies on cultured epithelial cells. Bacterial attachment and effacement of the enterocyte brush border membrane is attributed to a surface outer membrane protein adhesin termed intimin and EPEC-secreted proteins EspA, EspB, and EspD, Except for intimin, production in vivo or antibody response against these virulence determinants during natural EPEC infections in young children has not been demonstrated. Antibody responses against BfpA, intimin, EspA, and EspB were investigated in Brazilian children naturally infected with EPEC, Generally IgG antibodies against BfpA and EspB were the most commonly found, followed by anti-EspA and intimin antibodies. Thus, bundle-forming pilus and locus of enterocyte attachment-encoded products are produced in vivo during natural EPEC infections and elicit an immune response against heterologous EPEC virulence determinants. These findings have important implications in the immunoprophylaxis against EPEC infections.