Comparison of cellular ribonucleoprotein complexes associated with the APOBEC3F and APOBEC3G antiviral proteins
Comparison of cellular ribonucleoprotein complexes associated with the APOBEC3F and APOBEC3G antiviral proteins
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DOI：
10.1128/jvi.00287-08
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发表时间：
2008-06-01
影响因子：
5.4
通讯作者：
Malim, Michael H.
中科院分区：
文献类型：
--
作者：
Gallois-Montbrun, Sarah;Holmes, Rebecca K.;Malim, Michael H.
人载脂蛋白B基因编码酶催化多肽样3F(APOBEC3F[A3F])和A3G蛋白是多种逆转录元件感染的有效抑制因子，氨基酸序列同源性接近50%。因此，我们对A3F和A3G相关的核糖核蛋白复合体(RNPs)的蛋白质和RNA组成进行了比较分析。与A3G一样，A3F被发现与一系列复杂的细胞质RNPs相关，并可以在RNA丰富的细胞质微域中积累，称为mRNA处理体或应激颗粒。虽然A3F RNPs对RNase消化的破坏表现出更强的抵抗力，但主要的蛋白质差异是缺乏Ro60和La自身抗原。与此一致，A3F RNPs也缺乏一些小的聚合酶III RNA，包括与RoRNP相关的Y RNA以及7SL RNA。然而，Alu RNA存在于A3F和A3G RNPs中，这两种蛋白质都抑制了Alu元件的逆转录转座。因此，我们定义了与A3F和A3G相关的RNPs之间的一些细微差异，并推测这些差异有助于这些蛋白质的功能差异。
The human apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F (APOBEC3F [A3F]) and A3G proteins are effective inhibitors of infection by various retroelements and share similar to 50% amino acid sequence identity. We therefore undertook comparative analyses of the protein and RNA compositions of A3F- and A3G-associated ribonucleoprotein complexes (RNPs). Like A3G, A3F is found associated with a complex array of cytoplasmic RNPs and can accumulate in RNA-rich cytoplasmic microdomains known as mRNA processing bodies or stress granules. While A3F RNPs display greater resistance to disruption by RNase digestion, the major protein difference is the absence of the Ro60 and La autoantigens. Consistent with this, A3F RNPs also lack a number of small polymerase III RNAs, including the RoRNP-associated Y RNAs, as well as 7SL RNA. Alu RNA is, however, present in A3F and A3G RNPs, and both proteins suppress Alu element retrotransposition. Thus, we define a number of subtle differences between the RNPs associated with A3F and A3G and speculate that these contribute to functional differences that have been described for these proteins.