Arachidonic acid metabolism in cultured mouse keratinocytes.
Arachidonic acid metabolism in cultured mouse keratinocytes.
复制标题
培养的小鼠角质形成细胞中的花生四烯酸代谢。
DOI：
10.1111/1523-1747.ep12275349
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
H. Kanoh
中科院分区：
文献类型：
--
作者：
H. Kondoh;Y. Sato;H. Kanoh
我们试图描述培养的小鼠角质形成细胞中花生四烯酸代谢的一般特征。用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）、离子载体A23187和胎牛血清（FBS）刺激[3 H]花生四烯酸标记的细胞。共同的三种物质，磷脂酰肌醇，磷脂酰乙醇胺和磷脂酰胆碱几乎同样作为来源的花生四烯酸释放的磷脂酶A2的行动。观察到A23187对磷脂酶A2作用的刺激作用大于FBS大于TPA。当TPA或A23187刺激时，释放到细胞外介质中的放射性主要见于前列腺素（PG）E2。其他PG和羟基二十碳四烯酸（HETE）的形成非常有限。然而，在FBS刺激的情况下，释放的放射性主要与未转化的花生四烯酸有关。FBS还抑制TPA和A23187诱导的花生四烯酸向PGE 2的转化。三种刺激物诱导的磷脂降解同样依赖于细胞外Ca 2+。FBS和A23187的刺激被钙调素拮抗剂抑制，但与FBS相比，A23187的作用对拮抗剂更敏感。当一起测试时，我们观察到三种刺激物的相加效应。
We attempted to characterize the general features of arachidonate metabolism in cultured mouse keratinocytes. The cells labeled with [3H]arachidonate were stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA), ionophore A23187, and fetal bovine serum (FBS). Common to the three substances, phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine almost equally served as sources of arachidonate liberated by the action of phospholipase A2. The stimulation of phospholipase A2 action was observed in the order of A23187 greater than FBS greater than TPA. When stimulated by TPA or A23187, the radioactivity released into the extracellular medium was mostly found in prostaglandin (PG) E2. Formation of other PGs and hydroxyeicosatetraenoate (HETE) was extremely limited. In the case of stimulation by FBS, however, the released radioactivity was mainly associated with non-converted arachidonate. FBS also inhibited the TPA- and A23187-induced conversion of arachidonate to PGE2. Phospholipid degradation induced by the three stimulators was similarly dependent on extracellular Ca2+. The stimulation by FBS and A23187 was suppressed by calmodulin antagonists, though the effect of A23187 was much more sensitive to the antagonists when compared to that of FBS. We observed more than additive effects of the three stimulators when tested together.
登录
查看更多内容
表皮花生四烯酸脂氧合酶。
DOI：
10.1016/0005-2760(83)90295-3
发表时间：
1983
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Ruzicka,T;Vitto,A;Printz,MP
通讯作者：
Printz,MP
人中性粒细胞中花生四烯酸的释放和代谢。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Walsh,CE;Waite,BM;Thomas,MJ;DeChatelet,LR
通讯作者：
DeChatelet,LR
DOI：
10.1073/pnas.80.1.36
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
NIEDEL, JE;KUHN, LJ;VANDENBARK, GR
通讯作者：
VANDENBARK, GR
豚鼠皮肤中花生四烯酸的代谢。
DOI：
10.1016/0005-2760(82)90052-2
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Ruzicka,T;Printz,MP
通讯作者：
Printz,MP