Drosophila melanogaster mitochondrial DNA: Completion of the nucleotide sequence and evolutionary comparisons
Drosophila melanogaster mitochondrial DNA: Completion of the nucleotide sequence and evolutionary comparisons
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DOI：
10.1111/j.1365-2583.1995.tb00032.x
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发表时间：
1995-11-01
影响因子：
2.6
通讯作者：
Kaguni, LS
中科院分区：
文献类型：
--
作者：
Lewis, DL;Farr, CL;Kaguni, LS
测定了黑腹果蝇（Drosophilamelanogaster）线粒体DNA（mtDNA）A+T区侧翼区的核苷酸序列。包括编码缬氨酸、异亮氨酸、谷氨酰胺和甲硫氨酸的转移RNA、小核糖体RNA和大核糖体RNA的5 '编码序列以及NADH脱氢酶亚基II的基因。这就完成了D.线粒体基因组。D.黑腹果蝇的大小在不同种群间的差异很大程度上是由于控制区的长度差异（Fauron & Wolstenholme，1976; Fauron & Wolstenholme，1980 a，B）;我们测序的mtDNA区域与其他人测序的mtDNA区域相结合，产生了一个长度为19，517 bp的复合基因组，而黑腹果蝇的mtDNA为16，019 bp。日本料理。D.黑腹果蝇mtDNA的碱基组成有很大的偏差，其脱氧腺苷酸和胸苷酸残基的比例为82.2%，而果蝇为78.6%。雅库巴线粒体DNA这两个物种的线粒体DNA中编码的所有基因在相同的位置和方向。核苷酸替换分析表明，tRNA和rRNA基因的进化速度不到蛋白质编码基因的一半。
The nucleotide sequence of the regions flanking the A+T region of Drosophila melanogaster mitochondrial DNA (mtDNA) has been determined. Included are the genes encoding the transfer RNAs for valine, isoleucine, glutamine and methionine, the small ribosomal RNA and the 5'-coding sequences of the large ribosomal RNA and NADH dehydrogenase subunit II. This completes the nucleotide sequence of the D. melanogaster mitochondrial genome. The circular mtDNA of D. melanogaster varies in size among different populations largely due to length differences in the control region (Fauron & Wolstenholme, 1976; Fauron & Wolstenholme, 1980a, b); the mtDNA region we have sequenced, combined with those sequenced by others, yields a composite genome that is 19,517 bp in length as compared to 16,019 bp for the mtDNA of D. yakuba. D. melanogaster mtDNA exhibits an extreme bias in base composition; it comprises 82.2% deoxyadenylate and thymidylate residues as compared to 78.6% in D. yakuba mtDNA. Ail genes encoded in the mtDNA of both species are in identical locations and orientations. Nucleotide substitution analysis reveals that tRNA and rRNA genes evolve at less than half the rate of protein coding genes.