Role of ventral hippocampus in acquisition, consolidation and retrieval of rat's passive avoidance response memory trace
Role of ventral hippocampus in acquisition, consolidation and retrieval of rat's passive avoidance response memory trace
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DOI：
10.1016/s0006-8993(97)00651-3
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发表时间：
1997-09-12
期刊：
影响因子：
2.9
通讯作者：
Tassoni, G
中科院分区：
文献类型：
--
作者：
Lorenzini, CGA;Baldi, E;Tassoni, G
通过局部注射河豚毒素（TTX），获得了大鼠腹侧海马（VH）完全可逆的功能失活，以表征该结构在条件被动回避反应（PAR）记忆中的作用。在实验1中，对永久插管动物，分别在PAR获取前1小时、PAR获取后1小时和PAR获取前1小时在VH单侧或双侧注射TTX （1.0 μ l生理盐水中10 ng）或生理盐水（1.0 μ l），并在获取试验后48小时进行。结果表明，获取前和检索前VH单侧或双侧阻断均导致显著的PAR保留损伤，而获取后仅双侧阻断决定PAR保留损伤。在实验2中，在三组不同的大鼠身上，在全身氯胺酮麻醉（100 mg/kg b.w.）下，在不同的获取后延迟（0.25、1.5、6 h），双侧给药TTX （10 ng / 1 μ l生理盐水）。治疗后48小时的恢复测试显示，采集后双侧VH阻断仅在采集后0.25小时才会导致PAR损伤。结果清楚地表明，VH在PAR印痕的获取、巩固和检索过程中起着重要作用。实验证据讨论了TTX海马背侧阻断对大鼠PAR的影响，以及与海马与内侧间隔区和杏仁核的连通性的关系。(C) 1997爱思唯尔科学有限公司
By means of local administration of tetrodotoxin (TTX) a fully reversible functional inactivation of rat's ventral hippocampus (VH) was obtained in order to characterize the role of this structure in the memorization of a conditioned passive avoidance response (PAR). In Experiment 1, on permanently cannulated animals, TTX (10 ng in 1.0 mu l saline) or saline (1.0 mu l) was injected uni- or bilaterally in the VH, respectively, 1 h before PAR acquisition, immediately after PAR acquisition, and 1 h before PAR retrieval, always performed 48 h after the acquisition trial. It was shown that both pre-acquisition and pre-retrieval VH uni-or bilateral blockades were followed by significant PAR retention impairment, while in post-acquisition only the bilateral blockade determined PAR retention impairment. In Experiment 2, on three different groups of rats, TTX (10 ng in 1 mu l saline) was bilaterally administered, under general ketamine anesthesia (100 mg/kg b.w.), into the VH at different post-acquisition delays (0.25, 1.5, 6 h). Retrieval testing, 48 h after treatment, showed that post-acquisition bilateral VH blockade caused PAR impairment only when performed 0.25 h after acquisition. The results clearly indicate a role of VH during acquisition, consolidation and retrieval of PAR engram. The experimental evidence is discussed in comparison to previous results concerning TTX dorsal hippocampus blockade effects on rat's PAR and in relation to hippocampal connectivity with the medial septal area and the amygdala. (C) 1997 Elsevier Science B.V.