Endurance exercise training promotes medullary hematopoiesis
Endurance exercise training promotes medullary hematopoiesis
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DOI：
10.1096/fj.11-189043
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发表时间：
2011-12-01
期刊：
影响因子：
4.8
通讯作者：
Parise, Gianni
中科院分区：
文献类型：
--
作者：
Baker, J. M.;De Lisio, Michael;Parise, Gianni
耐力运动是一种定义不明确但功能强大的造血调节剂。本研究的目的是直接研究耐力运动训练对造血的影响，并确定导致任何观察到的变化的潜在机制。 4 周大的雄性 C57Bl/6 小鼠在跑步机上进行为期 10 周的渐进速度训练。最后一次训练后 2 天收获组织。使用流式细胞术、鹅卵石区域形成细胞测定和甲基纤维素集落形成单位测定来评估髓质和动员的造血干细胞和祖细胞。使用定量实时 PCR 和蛋白质印迹来测量造血细胞因子的产生。组织化学也被用来评估骨髓生态位对运动的适应。根据细胞类型，耐力训练可将髓质和动员的造血干细胞和祖细胞含量从 50% 增加到 800%。训练还使骨髓腔脂肪减少了 78%。通过训练，骨骼肌造血细胞因子的表达也增加了至少 60%。久坐的小鼠作为上述实验的对照。总之，耐力运动训练极大地促进造血，这是通过改善髓质生态位结构以及增加骨骼肌造血细胞因子的产生来实现的。-Baker，J. M.，De Lisio，M.，Parise，G。耐力运动训练促进髓质造血。 FASEB J. 25, 4348-4357 (2011)。 www.fasebj.org
Endurance exercise is a poorly defined yet powerful mediator of hematopoiesis. The purpose of this study was to directly investigate the effects of endurance exercise training on hematopoiesis and to identify potential mechanisms responsible for any observed changes. Four-week-old male C57Bl/6 mice were trained on a treadmill at progressive speeds over a 10-wk period. Tissues were harvested 2 d following the final training session. Flow cytometry, the cobblestone area-forming cell assay, and the methycellulose colony-forming unit assay were used to assess medullary and mobilized hematopoietic stem and progenitor cells. Quantitative real-time PCR and Western blots were used to measure hematopoietic cytokine production. Histochemistry was also used to assess adaptations to exercise in the bone marrow niche. Depending on the cell type, endurance training increased medullary and mobilized hematopoietic stem and progenitor cell content from 50 to 800%. Training also reduced marrow cavity fat by 78%. Skeletal muscle hematopoietic cytokine expression was also increased at least 60% by training. Sedentary mice served as controls for the above experiments. In conclusion, endurance exercise training greatly promotes hematopoiesis and does so through improvements in medullary niche architecture as well as increased skeletal muscle hematopoietic cytokine production.-Baker, J. M., De Lisio, M., Parise, G. Endurance exercise training promotes medullary hematopoiesis. FASEB J. 25, 4348-4357 (2011). www.fasebj.org