OVARIECTOMY-INDUCED BONE LOSS AND THE HEMATOPOIETIC SYSTEM
OVARIECTOMY-INDUCED BONE LOSS AND THE HEMATOPOIETIC SYSTEM
复制标题
DOI：
10.1016/s0169-6009(08)80050-5
复制
发表时间：
1993-11-01
期刊：
影响因子：
--
通讯作者：
SALIH, MA
中科院分区：
文献类型：
--
作者：
KALU, DN;SALERNO, E;SALIH, MA
为了研究造血系统与卵巢激素缺乏引起的骨丢失的关系，我们检测了卵巢切除术和雌激素给药对胸腺、脾脏和骨髓的影响，以及对破骨细胞骨髓祖细胞增殖的影响。我们还评估了每日给予白细胞介素-1受体拮抗剂（IL-1 ra）对卵巢激素缺乏引起的骨丢失的影响。卵巢切除术导致松质骨体积减少，小梁成骨细胞和破骨细胞数量增加，血清碱性磷酸酶水平升高，17 β-雌二醇治疗可预防这些变化。卵巢切除后，胸腺重量、脾脏重量、胸腺和脾脏淋巴细胞以及骨髓单核细胞和淋巴细胞也显著增加，并且这些增加被17 β-雌二醇抑制。卵巢切除术，此外，造成了4倍的抗酒石酸酸性磷酸酶（TRAP）阳性多核细胞的骨髓细胞培养物中形成的数量增加，增加部分抑制17 β-雌二醇。IL-1 ra给药不能预防卵巢切除术引起的骨丢失。我们的研究结果表明，卵巢切除引起的骨质流失的大鼠伴随着造血系统的显着变化，这些变化是由雌激素管理调制。尽管IL-1 ra检测结果为阴性，但造血系统参与卵巢激素缺乏所致骨丢失发病机制的性质值得继续探讨。
To investigate the relationship of the hematopoietic system to the loss of bone due to ovarian hormone deficiency, we examined the effects of ovariectomy and estrogen administration on the thymus, spleen and the bone marrow, and on the proliferation of marrow progenitor's of osteoclasts. We also assessed the effects of daily administration of interleukin-l receptor antagonist (IL-1ra) on bone loss due to ovarian hormone deficiency. Ovariectomy resulted in decreased cancellous bone volume, increased trabecular osteoblast and osteoclast numbers, and increased serum alkaline phosphatase levels that were prevented by 17 beta-estradiol treatment. Thymus weight, spleen weight, thymus and spleen lymphocytes, and bone marrow monocytes and lymphocytes also increased significantly following ovariectomy, and the increases were suppressed by 17 beta-estradiol. Ovariectomy, in addition, caused a 4-fold increase in the number of tartrate resistant acid phosphatase (TRAP)-positive multinucleated cells formed in cultures of marrow cells and the increase was partially inhibited by 17 beta-estradiol. IL-1ra administration did not prevent the bone loss due to ovariectomy. Our findings indicate that ovariectomy-induced bone loss in the rat is accompanied by marked changes in the hematopoietic system, and that these changes are modulated by estrogen administration. In spite of the negative finding with IL-1ra, the nature of the involvement of the hematopoietic system in the pathogenesis of bone loss due to ovarian hormone deficiency merits continued exploration.