Electroacupuncture enhances motor recovery performance with brain-derived neurotrophic factor expression in rats with cerebral infarction
Electroacupuncture enhances motor recovery performance with brain-derived neurotrophic factor expression in rats with cerebral infarction
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DOI：
10.1136/acupmed-2011-010126
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Kim, Manho
中科院分区：
文献类型：
--
作者：
Kim, Min-Wook;Chung, You Chul;Kim, Manho
目的：电针是一种传统医学对脑卒中患者康复的方法。脑源性神经营养因子（BDNF）是一种有效的生长因子，参与脑损伤后的恢复。本研究的目的是探讨是否EA增加BDNF的水平和促进功能recovery.Methods闭塞的大脑中动脉在大鼠（N = 12），然后再灌注。电针治疗组：针刺GV 20（百会）穴。运动和感觉功能监测加西亚规模为2周。结果电针组大鼠的Garcia评分，尤其是运动成绩明显改善（p <0.05），而电针组大鼠的运动成绩明显改善（p < 0.05）。在电针的作用下，缺血半球的BDNF升高，BDNF（+）细胞数量增加。结论电针GV 20可促进脑缺血大鼠运动功能恢复，并促进BDNF/trkB的表达。
Objective Electroacupuncture (EA) is a traditional medicine in patients with post-stroke rehabilitation. Brain-derived neurotrophic factor (BDNF) is a potent growth factor involved in recovery following cerebral injury. The aim of the present study was to investigate whether EA increases BDNF levels and facilitates functional recovery.Methods Occlusion of the middle cerebral artery was performed in rats (N = 12) followed by reperfusion. EA was applied at the GV20 (Baihui) acupoint. Motor and sensory functions were monitored on the Garcia scale for 2 weeks. Expressions of BDNF and receptor tyrosine kinase B (trkB) were determined by immunoblotting and immunohistochemistry.Results Improvement of Garcia scores, particularly in motor performance, were noted in the group with EA stimulation (p < 0.05). With EA application, BDNF was elevated in the ischaemic hemisphere with increased numbers of BDNF(+) cells. Increased expression of trkB was also detected.Conclusion These results indicate that EA at GV20 improves motor recovery and stimulates BDNF/trkB expression in rats with cerebral ischaemia.