Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha 2/delta subunit
Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha 2/delta subunit
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DOI：
10.1016/s0014-5793(96)01205-7
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发表时间：
1996-11-18
期刊：
影响因子：
3.5
通讯作者：
Hofmann, F
中科院分区：
文献类型：
--
作者：
Angelotti, T;Hofmann, F
用文库筛选和RT-PCR相结合的方法克隆了小鼠α2/β亚基亚基不同亚型(α2a-e)的5种不同剪接变异体，通过RNase保护实验确定了各种亚型在小鼠组织中的表达模式和相对丰度，骨骼肌和脑分别表达单一亚型α2a和α2b；然而，心血管系统表达所有这五种亚型，心脏主要表达α2c和α2d，而与其他物种不同，主动脉主要表达‘骨骼肌’亚型。含有平滑肌的组织表达α2d和α2e。因此，α2/Delta亚型在组织中的表达受到限制，这表明差异剪接变异体具有重要的功能作用。
Five different splice variants of mouse alpha 2/delta subunit isoforms (alpha 2a-e), which arose from various combinations of three alternatively spliced regions, were cloned with a combination of cDNA library screening and RT-PCR, Expression patterns and relative abundance of the various isoforms in mouse tissues were determined with an RNAse protection assay, Skeletal muscle and brain expressed single isoforms, alpha 2a and alpha 2b, respectively; however, the cardiovascular system expressed all five isoforms, Heart expressed mainly isoforms alpha 2c and alpha 2d while, in contrast to other species, aorta expressed predominantly alpha 2a, the 'skeletal muscle' isoform. Smooth muscle-containing tissues expressed alpha 2d and alpha 2e. Thus, alpha 2/delta isoforms are restricted in their tissue expression, suggesting an important functional role for the differentially spliced variants.