New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides
New Insights Into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous Orange-Spotted Grouper, Epinephelus coioides
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卵泡刺激激素在雌性橙斑石斑鱼（斜带石斑鱼）性别分化中作用的新见解
DOI：
10.3389/fendo.2019.00304
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发表时间：
2019-05-15
影响因子：
5.2
通讯作者：
Zhang, Yong
中科院分区：
文献类型：
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作者：
Huang, Minwei;Chen, Jiaxing;Zhang, Yong
卵泡刺激素（FSH）信号被认为对硬骨鱼的早期配子发生至关重要，但其在性别分化中的功能作用在很大程度上是未知的。在本研究中，我们研究了长期和短期注射卵泡刺激素对原雌石斑鱼（Epinephelus coioides）性别分化的影响。长期FSH治疗最初促进了卵巢的形成，但随后诱导了男性的命运。雌性途径基因的表达先升高后降低，而雄性途径基因的表达仅在长期FSH处理期间上调。与性类固醇激素合成相关的基因，以及血清11-酮睾酮和雌二醇，也在长期FSH治疗期间上调。短期FSH治疗在低剂量下激活了雌性通路中的基因（尤其是cyp19a1a），但在高剂量下引起抑制。高浓度的卵泡刺激素在短期内上调了雄性途径的基因。最后，我们发现低浓度（而不是高浓度）FSH处理激活了人胚胎肾（HEK） 293细胞中cyp19a1a启动子的活性。总的来说，我们的数据表明，FSH可能诱导雌性橙斑石斑鱼卵巢分化或改变雄性命运，这些过程以FSH浓度依赖的方式发生。
Follicle-stimulating hormone (FSH) signaling is considered to be essential for early gametogenesis in teleosts, but its functional roles during sex differentiation are largely unknown. In this study, we investigated the effects of long-term and short-term FSH injection on sex differentiation in the protogynous orange-spotted grouper (Epinephelus coioides). Long-term FSH treatment initially promoted the formation of ovaries but subsequently induced a male fate. The expression of female pathway genes was initially increased but then decreased, whereas the expression of male pathway genes was up-regulated only during long-term FSH treatment. The genes related to the synthesis of sex steroid hormones, as well as serum 11-ketotestosterone and estradiol, were also up-regulated during long-term FSH treatment. Short-term FSH treatment activated genes in the female pathway (especially cyp19a1a) at low doses but caused inhibition at high doses. Genes in the male pathway were up-regulated by high concentrations of FSH over the short term. Finally, we found that low, but not high, concentrations of FSH treatment activated cyp19a1a promoter activities in human embryonic kidney (HEK) 293 cells. Overall, our data suggested that FSH may induce ovarian differentiation or a change to a male sex fate in the protogynous orange-spotted grouper, and that these processes occurred in an FSH concentration-dependent manner.