Assessing the Collective Dynamics of Motile Cilia in Cultures of Human Airway Cells by Multiscale DDM
Assessing the Collective Dynamics of Motile Cilia in Cultures of Human Airway Cells by Multiscale DDM
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DOI：
10.1016/j.bpj.2017.05.028
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发表时间：
2017-07-11
影响因子：
3.4
通讯作者：
Cicuta, Pietro
中科院分区：
文献类型：
--
作者：
Feriani, Luigi;Juenet, Maya;Cicuta, Pietro
差分动态显微镜技术在这里扩展，表明它可以提供一个强大的和客观的方法，视频分析的活的人支气管上皮纤毛细胞的体外样品的光学显微镜视频。这些细胞是多纤毛的，具有发挥关键生理作用的运动纤毛。结果表明，纤毛拍频可以恢复到传统的分析相匹配，但在一个完全自动化的方式。此外，它示出的纤毛节拍的空间和时间的相干性的属性可以恢复和区分，并且，如果集体行波（异时性波）存在，这有一个独特的签名，其波长和方向可以测量。
The technique of differential dynamic microscopy is extended here, showing that it can provide a powerful and objective method of video analysis for optical microscopy videos of in vitro samples of live human bronchial epithelial ciliated cells. These cells are multiciliated, with motile cilia that play key physiological roles. It is shown that the ciliary beat frequency can be recovered to match conventional analysis, but in a fully automated fashion. Furthermore, it is shown that the properties of spatial and temporal coherence of cilia beat can be recovered and distinguished, and that if a collective traveling wave (the metachronal wave) is present, this has a distinct signature and its wavelength and direction can be measured.