The immunogenic properties of protozoan glycosylphosphatidylinositols in the mosquito Anopheles gambiae
The immunogenic properties of protozoan glycosylphosphatidylinositols in the mosquito Anopheles gambiae
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DOI：
10.1016/j.dci.2008.08.009
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Faye, Ingrid
中科院分区：
文献类型：
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作者：
Arrighi, Romanico B. G.;Debierre-Grockiege, Francoise;Faye, Ingrid
与人类不同，蚊子没有适应性免疫反应来对付病原体，因此必须依靠它们的先天免疫系统来对付入侵者。这有助于基于表面组分或抗原识别不同的微生物。已经在各种类型的微生物如细菌和真菌中鉴定出这样的抗原，但是在原生动物属中还没有鉴定出这样的抗原，原生动物属包括病原体如疟疾寄生虫、恶性疟原虫和刚地弓形虫。这项研究使我们能够测试原生动物糖基磷脂酰肌醇（GPI）对蚊子免疫系统的抗原特性。我们发现，恶性疟原虫GPI和弓形虫GPI在摄入后诱导几种抗微生物肽的强烈表达，并且作为针对GPI的免疫应答的结果，蚊子产生的卵的数量显著减少。这种效应与感染疟疾的蚊子有关，但从未与疟原虫特异性抗原有关。这项研究表明，原生动物GPI可以被认为是蚊子中的原生动物特异性免疫激发子，并且恶性疟原虫GPI在疟原虫操纵蚊子载体以促进其传播中起着关键作用。(C)2008爱思唯尔有限公司保留所有权利。
In contrast to humans, mosquitoes do not have an adaptive immune response to deal with pathogens, and therefore must rely on their innate immune system to deal with invaders. This facilitates the recognition of different microbes on the basis of surface components or antigens. Such antigens have been identified in various types of microbe such as bacteria and fungi, yet none has been identified in the genus protozoa, which includes pathogens such as the malaria parasite, Plasmodium falciparum and Toxoplasma gondii. This study allowed us to test the antigenic properties of protozoan glycosylphosphatidylinositol (GPI) on the mosquito immune system. We found that both P. falciparum GPI and T gondii GPI induce the strong expression of several antimicrobial pepticles following ingestion, and that as a result of the immune response against the GPIs, the number of eggs produced by the mosquito is reduced dramatically. Such effects have been associated with malaria infected mosquitoes, but never associated with a Plasmodium specific antigen. This study demonstrates that protozoan GPIs can be considered as protozoan specific immune elicitors in mosquitoes, and that P. falciparum GPI plays a critical role in the malaria parasite manipulation of the mosquito vector to facilitate its transmission. (C) 2008 Elsevier Ltd. All rights reserved.