Oligoclonal T cell repertoire in cerebrospinal fluid of patients with inflammatory diseases of the nervous system
Oligoclonal T cell repertoire in cerebrospinal fluid of patients with inflammatory diseases of the nervous system
复制标题
DOI：
10.1136/jnnp.70.6.767
复制
发表时间：
2001-06-01
影响因子：
11
通讯作者：
Massacesi, L
中科院分区：
文献类型：
--
作者：
Gestri, D;Baldacci, L;Massacesi, L
目的：研究自身免疫性和炎症性神经系统疾病患者脑脊液细胞和外周血单核细胞中T细胞受体P链可变区(TCRBV)基因的体外应用。方法：建立了一种新的灵敏的半套式聚合酶链式反应和异源双链分析方法。结果在本实验条件下，建立了用于分析整个T细胞的最小细胞数为2.5×10(4)-足以评价大多数诊断腰椎穿刺术中采集的样本。在接受检查的21名患者中，TCRBV基因家族的使用没有受到限制。然而，通过异源双链分析，13例患者的脑脊液中发现了寡克隆性T细胞的扩增，5例患者的脑脊液中发现了单克隆性的T细胞扩增。结论T细胞克隆性扩增有助于神经系统炎症过程中病理性T细胞的进一步定性，但这种异常可能不是疾病所特有的。
Objective - To evaluate the T cell receptor p chain variable region (TCRBV) gene usage ex vivo in CSF cells and peripheral blood mononuclear cells (PBMCs) collected from patients with autoimmune and inflammatory diseases of the nervous system.Methods - A novel sensitive seminested-polymerase chain reaction coupled with heteroduplex analysis was developed.Results - Under these experimental conditions, the minimal number of cells required for the analysis of the whole T cell repertoire was established at 2.5x10(4)-suffcient to evaluate most of the samples collected during diagnostic lumbar punctures. In the 21 patients examined, restrictions in TCRBV gene family usage were not seen. However, using heteroduplex analysis, oligoclonal T cell expansions were found in the CSF of 13 patients and monoclonal expansions in five patients. The T cell abnormalities found did not correlate with intrathecal IgG production or with any clinical variable considered.Conclusion - T cell clonal expansions, useful for further characterisation of pathogenetic T cells, can be found during the course of nervous system inflammations, but this abnormality is probably not disease specific.