Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile
Mechanism of Antibacterial Activity of Bacillus amyloliquefaciens C-1 Lipopeptide toward Anaerobic Clostridium difficile
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解淀粉芽孢杆菌C-1脂肽对厌氧艰难梭菌的抗菌作用机制
DOI：
10.1155/2020/3104613
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发表时间：
2020-03-04
影响因子：
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通讯作者：
Han, Bei
中科院分区：
文献类型：
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作者：
Lv, Jia;Da, Rong;Han, Bei
益生菌可能为治疗艰难梭菌感染(CDI)的标准抗生素疗法提供一个有吸引力的替代方案。本研究对新分离的解淀粉芽孢杆菌C-1对艰难梭菌的体外抗菌机制进行了研究。C-1菌株产生的脂肽表面素、伊图灵和风霉素对艰难梭菌的生长和存活有很强的抑制作用。对其抑菌机理的系统研究表明，C-1脂肽破坏艰难梭菌细胞壁和细胞膜的完整性。此外，脂肽与艰难梭菌基因组DNA结合，导致细胞死亡。基因组重测序发现了许多重要的抗菌化合物编码簇，包括6个非核糖体多肽(表面蛋白(SrfABCD)、iturins(ItuABCD)、fengycins(FenABCDE)、杆状杆菌蛋白(BmyABC)、teichuronic和bacilysin)和3个聚酮(bacillaene(BaeEDLMNJRS)、DifABCDEFGHIJ和大乳酸蛋白(MlnABCDEFGHI))。此外，还有其他有益的基因，如磷脂酶和7个铁载体生物合成基因簇，这些基因可能对淀粉液化芽孢杆菌C-1的抗菌活性有协同作用。我们认为，在艰难梭菌的控制中，适当应用抗菌肽可能是有效的。
Probiotics may offer an attractive alternative for standard antibiotic therapy to treat Clostridium difficile infections (CDI). In this study, the antibacterial mechanism in vitro of newly isolated B. amyloliquefaciens C-1 against C. difficile was investigated. The lipopeptides surfactin, iturin, and fengycin produced by C-1 strongly inhibited C. difficile growth and viability. Systematic research of the bacteriostatic mechanism showed that the C-1 lipopeptides damage the integrity of the C. difficile cell wall and cell membrane. In addition, the lipopeptide binds to C. difficile genomic DNA, leading to cell death. Genome resequencing revealed many important antimicrobial compound-encoding clusters, including six nonribosomal peptides (surfactins (srfABCD), iturins (ituABCD), fengycins (fenABCDE), bacillibactin (bmyABC), teichuronic, and bacilysin) and three polyketides (bacillaene (baeEDLMNJRS), difficidin (difABCDEFGHIJ), and macrolactin (mlnABCDEFGHI)). In addition, there were other beneficial genes, such as phospholipase and seven siderophore biosynthesis gene clusters, which may contribute synergistically to the antibacterial activity of B. amyloliquefaciens C-1. We suggest that proper application of antimicrobial peptides may be effective in C. difficile control.