Molecular Cytogenetic Characterization of the Antirrhinum majus Genome Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under the accession nos. AY630561 (for BAC 5E10) and AY6305612 (for BAC 36D21).
Molecular Cytogenetic Characterization of the Antirrhinum majus Genome Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under the accession nos. AY630561 (for BAC 5E10) and AY6305612 (for BAC 36D21).
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DOI：
10.1534/genetics.104.031146
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发表时间：
2005-01
期刊：
影响因子：
3.3
通讯作者：
Dongfen Zhang;Qiuying Yang;Weidong Bao;Yu Zhang;B. Han;Yongbiao Xue;Zhukuan Cheng
中科院分区：
文献类型：
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作者：
Dongfen Zhang;Qiuying Yang;Weidong Bao;Yu Zhang;B. Han;Yongbiao Xue;Zhukuan Cheng
金鱼草属植物作为经典植物遗传学的模式系统，在配子体自交不亲和性、花发育生物学和转座子诱导突变等方面的研究已取得了很大进展。与遗传和分子研究的进展相反，对金鱼草细胞遗传学知之甚少。在这项研究中，我们分离出两个串联重复序列，CentA1和CentA2，从金鱼草染色体的着丝粒区域。利用FISH技术将这些着丝粒重复序列锚定在减数分裂粗线期染色体上，建立了标准的核型。根据粗线期染色体的DAPI染色模式，建立了一个描述金鱼草基因组中异染色质分布的表意图。为了整合遗传图谱和染色体图谱，我们从每个连锁群中选择一个或两个分子标记来筛选金鱼草可转化人工染色体（TAC）文库。将这些基因锚定的TAC克隆标记为FISH探针，与A. majus。从而建立了金鱼草属植物染色体与连锁群的关系。
As a model system in classical plant genetics, the genus Antirrhinum has been well studied, especially in gametophytic self-incompatibility, flower development biology, and transposon-induced mutation. In contrast to the advances in genetic and molecular studies, little is known about Antirrhinum cytogenetics. In this study, we isolated two tandem repetitive sequences, CentA1 and CentA2, from the centromeric regions of Antirrhinum chromosomes. A standard karyotype has been established by anchoring these centromeric repeats on meiotic pachytene chromosome using FISH. An ideogram based on the DAPI-staining pattern of pachytene chromosomes was developed to depict the distribution of heterochromatin in the Antirrhinum majus genome. To integrate the genetic and chromosomal maps, we selected one or two molecular markers from each linkage group to screen an Antirrhinum transformation-competent artificial chromosome (TAC) library. These genetically anchored TAC clones were labeled as FISH probes to hybridize to pachytene chromosomes of A. majus. As a result, the relationship between chromosomes and the linkage groups (LGs) in Antirrhinum has been established.