Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.
Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed.
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DOI：
10.3390/ijms17040507
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发表时间：
2016-04-06
影响因子：
5.6
通讯作者：
Lin JT
中科院分区：
文献类型：
--
作者：
Chen GQ;van Erp H;Martin-Moreno J;Johnson K;Morales E;Browse J;Eastmond PJ;Lin JT
莱斯克雷氏菌是一种潜在的工业油料作物，可生产羟基脂肪酸(HFA)。与蓖麻不同，它的种子无毒，但主要在三酰甘油(TAG)的sn-1和sn-3位积累麦壳酸，而蓖麻在这三个位置都含有蓖麻酸(18：1OH)。为了研究莱斯奎拉菌是否可以在sn-2位置积累HFA，建立了多个在种子中表达蓖麻溶血磷脂酸酰基转移酶2(RcLPAT2)的转基因株系。RcLPAT2在标签的sn-2位增加了18：1OH，从2%增加到14%-17%，导致tri-HFA-标签从5%增加到13%-14%。我们的结果是第一个使用LPAT在种子标签的sn-2位增加蓖麻酸的例子。这项工作为莱斯克雷氏菌中含有HFA的标签组装的机制提供了见解，并指导了未来的研究，以优化该工厂生产HFA。
Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.