E-cadherin in the assessment of aberrant placental cytotrophoblast turnover in pregnancies complicated by pre-eclampsia
E-cadherin in the assessment of aberrant placental cytotrophoblast turnover in pregnancies complicated by pre-eclampsia
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DOI：
10.1007/s00418-005-0051-7
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发表时间：
2005-12-01
影响因子：
2.3
通讯作者：
Crocker, IP
中科院分区：
文献类型：
--
作者：
Brown, LM;Lacey, HA;Crocker, IP
E-钙粘蛋白是细胞滋养层中表达的细胞-细胞粘附蛋白，其在细胞滋养层分化和合胞化时丢失。我们已经利用E-钙粘蛋白作为细胞滋养层细胞的标志物，调查绒毛组织组成的第一和第三孕期胎盘，无论是在正常妊娠和妊娠并发先兆子痫。我们已经实现了这一点，通过测量表达水平的E-钙粘蛋白在mRNA水平，使用Q-PCR，并在蛋白质水平，使用半定量Western印迹。我们还结合E-钙粘蛋白免疫组织化学与面积测量的形态学分析，以确定细胞滋养层和合体滋养层的隔间。E-cadherin的这种新用途揭示了随着妊娠的进展，绒毛组织中细胞滋养细胞的比例减少，而合体滋养细胞覆盖没有变化。此外，在先兆子痫，胎盘E-钙粘蛋白升高相比，合体滋养层，这表明无论是夸张的细胞滋养层增殖或受损的细胞滋养层分化，这两个潜在的致病重要性的改变。
E-cadherin is a cell-cell adhesion protein expressed in cytotrophoblasts, which is lost as they differentiate and syncytialise. We have exploited E-cadherin as a marker of cytotrophoblasts to investigate villous tissue composition in first and third trimester placentae, both in normal pregnancy and pregnancies complicated by pre-eclampsia. We have achieved this by measuring expression levels of E-cadherin at the mRNA level, using Q-PCR, and at the protein level using semi-quantitative Western blotting. We have also combined E-cadherin immunohistochemistry with morphometric analysis of area measurements to define cytotrophoblast and syncytiotrophoblast compartments. This novel use of E-cadherin has revealed a decrease in the proportion of cytotrophoblasts in villous tissue as pregnancy progresses, in the absence of changes in syncytiotrophoblast cover. Moreover, in pre-eclampsia, placental E-cadherin was raised compared to syncytiotrophoblast, suggesting either exaggerated cytotrophoblast proliferation or impaired cytotrophoblast differentiation, both alterations of potential pathogenic importance.