Comprehensive Characterization of Nanosized Extracellular Vesicles from Central and Peripheral Organs : Implications for Preclinical and Clinical Applications.
Comprehensive Characterization of Nanosized Extracellular Vesicles from Central and Peripheral Organs : Implications for Preclinical and Clinical Applications.
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DOI：
10.1021/acsanm.0c01654
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发表时间：
2020-09-25
影响因子：
5.9
通讯作者：
Yelamanchili SV
中科院分区：
文献类型：
--
作者：
Chand S;Jo A;Vellichirammal NN;Gowen A;Guda C;Schaal V;Odegaard K;Lee H;Pendyala G;Yelamanchili SV
细胞外囊泡（EV）是纳米尺寸的囊泡，其作为用于多种病理学的潜在诊断标记物和治疗载体的有价值的作用已经获得了很多关注。虽然来自生物流体（例如血浆、血清、尿液、脑脊液和基于体外细胞培养的平台）的EV标志物已被广泛研究，但仍然存在的显著知识缺口是特定器官衍生EV（ODE）的表征。在这里，我们提出了一个标准化的协议，从大鼠和死后人体组织的脑，心脏，肺，肾脏和肝脏分离纯化EV的分离和表征。接下来，使用基于定量质谱的蛋白质组学，我们表征了相应的组织EV蛋白质组，所述蛋白质组将突触泡蛋白（SYP）、小窝蛋白-3（CAV 3）、溶质载体家族22成员2（SLC 22 A2）、表面活性蛋白B（SP-B）和脂肪酸结合蛋白1（FABP 1）分别鉴定为脑、心脏、肾、肺和肝EV的潜在标志物。在两个物种中使用免疫印迹和纳米等离子体平台-单EV成像分析进一步验证这些各自的组织特异性标志物。总之，我们的研究首次使用传统的生物化学和高精度技术平台，在定义特定的ODE标记物方面提供了有价值的概念验证方法，这些标记物可以进一步开发为相应终末器官相关病理的潜在治疗候选物。
Extracellular vesicles (EV) are nano-sized vesicles that have been garnering a lot of attention for their valuable role as potential diagnostic markers and therapeutic vehicles for a plethora of pathologies. Whilst EV markers from biofluids such as plasma, serum, urine, cerebrospinal fluid and in vitro cell culture based platforms have been extensively studied, a significant knowledge gap that remains is the characterization of specific organ derived EVs (ODE). Here, we present a standardized protocol for isolation and characterization of purified EV isolated from brain, heart, lung, kidney and liver from rat and postmortem human tissue. Next, using quantitative mass spectrometry based proteomics, we characterized the respective tissue EV proteomes that identified synaptophysin (SYP), caveolin-3 (CAV3), solute carrier family 22 member 2 (SLC22A2), surfactant protein B (SP-B), and fatty acid-binding protein 1 (FABP1) as potential markers for the brain, heart, kidney, lung, and liver-EV, respectively. These respective tissue specific markers were further validated using both immunoblotting and a nanoplasmonic platform- single EV imaging analysis in the two species. To summarize, our study for the first time using traditional biochemical and high precision technology platforms provide a valuable proof of concept approach in defining specific ODE markers which further could be developed as potential therapeutic candidates for respective end-organ associated pathologies.
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影响因子：
17.1
作者：
Lee K;Fraser K;Ghaddar B;Yang K;Kim E;Balaj L;Chiocca EA;Breakefield XO;Lee H;Weissleder R
通讯作者：
Weissleder R
DOI：
10.1073/pnas.1521230113
发表时间：
2016-02-23
影响因子：
11.1
作者：
Kowal, Joanna;Arras, Guillaume;Thery, Clotilde
通讯作者：
Thery, Clotilde
影响因子：
1.2
作者：
Ishiguro, Kaori;Yen, Irene K.;Patel, Tushar
通讯作者：
Patel, Tushar
影响因子：
23.9
作者：
Jacob A;Morley M;Hawkins F;McCauley KB;Jean JC;Heins H;Na CL;Weaver TE;Vedaie M;Hurley K;Hinds A;Russo SJ;Kook S;Zacharias W;Ochs M;Traber K;Quinton LJ;Crane A;Davis BR;White FV;Wambach J;Whitsett JA;Cole FS;Morrisey EE;Guttentag SH;Beers MF;Kotton DN
通讯作者：
Kotton DN
影响因子：
13.6
作者：
Gonzales, Patricia A.;Pisitkun, Trairak;Knepper, Mark A.
通讯作者：
Knepper, Mark A.