Tracking Hyaluronan: Molecularly Imprinted Polymer Coated Carbon Dots for Cancer Cell Targeting and Imaging
Tracking Hyaluronan: Molecularly Imprinted Polymer Coated Carbon Dots for Cancer Cell Targeting and Imaging
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DOI：
10.1021/acsami.7b16225
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发表时间：
2018-01-31
影响因子：
9.5
通讯作者：
Haupt, Karsten
中科院分区：
文献类型：
--
作者：
Demir, Bilal;Lemberger, Michael M.;Haupt, Karsten
抗癌战争不断需要新的亲和力工具来选择性地检测、定位和量化用于诊断或预后的生物标记物。在这里，碳纳米点(CDS)，一类新兴的荧光纳米材料，与分子印迹聚合物(MIP)相结合，被用作探测癌症生物标记物的生物相容性光学成像工具。首先，以淀粉为碳源，色氨酸为氮原子供给体，采用水热合成法制备了N掺杂的硫化镉，获得了25.1+/-2%的高量子产率。在紫外光的激发下，硫化镉的典型尺寸约为3.2 nm，并在450 nm处产生强烈的荧光。然后，利用CDS的发光作为光聚合的内部光源，在CDS周围合成了用于识别葡萄糖醛酸(GLCA)的MIP壳。GLCA是透明质酸的亚结构(表位)，透明质酸是某些癌症的生物标记物。利用CD核-MIP壳纳米复合材料对固定化的人宫颈癌细胞进行了透明质酸的生物靶向和生物成像。人角质形成细胞被用作非癌性参考细胞，确实，CD-MIP观察到的染色较少。
War against cancer constantly requires new affinity tools to selectively detect, localize, and quantify biomarkers for diagnosis or prognosis. Herein, carbon nanodots (CDs), an emerging class of fluorescent nanomaterials, coupled with molecularly imprinted polymers (MIPs), are employed as a biocompatible optical imaging tool for probing cancer biomarkers. First, N-doped CDs were prepared by hydrothermal synthesis using starch as carbon source and Ltryptophan as nitrogen atom provider to achieve a high quantum yield of 25.1 +/- 2%. The CDs have a typical size of similar to 3.2 nm and produce an intense fluorescence at 450 nm upon excitation with UV light. A MIP shell for specific recognition of glucuronic acid (GlcA) was then synthesized around the CDs, using the emission of the CDs as an internal light source for photopolymerization. GlcA is a substructure (epitope) of hyaluronan, a biomarker for certain cancers. The biotargeting and bioimaging of hyaluronan on fixated human cervical cancer cells using CD core-MIP shell nanocomposites is demonstrated. Human keratinocytes were used as noncancerous reference cells and indeed, less staining was observed by the CD-MIP.