Crypt Cells of the Zebrafish Danio rerio Mainly Project to the Dorsomedial Glomerular Field of the Olfactory Bulb
Crypt Cells of the Zebrafish Danio rerio Mainly Project to the Dorsomedial Glomerular Field of the Olfactory Bulb
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DOI：
10.1093/chemse/bjr109
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发表时间：
2012-05-01
期刊：
影响因子：
3.5
通讯作者：
Jesus Manso, Maria
中科院分区：
文献类型：
--
作者：
Gayoso, Jose;Castro, Antonio;Jesus Manso, Maria
斑马鱼的嗅粘膜由3种形态学类型的嗅觉受体神经元（ORN）组成：纤毛细胞、微绒毛细胞和隐窝细胞。以往的研究表明，在斑马鱼的纤毛和微绒毛的ORN的差异投影，这项目到不同的肾小球领域。然而，没有确定斑马鱼隐窝细胞的延髓靶点。在这里，我们分析了嗅上皮细胞和斑马鱼嗅球的背侧肾小球领域之间的关系，以及威尔斯这些球区域和前脑区域之间的连接。为此，在固定的组织中使用亲脂性碳菁示踪剂（DiI）。DiI在背内侧肾小球区的应用主要标记斑马鱼嗅上皮中的隐窝细胞。相比之下，DiI应用于肾小球背外侧区主要标记双极ORN，偶尔只标记隐窝细胞。球传出细胞（二尖瓣细胞）接触这些背侧肾小球领域项目到不同的端脑区，背侧端脑区（DP）的后区作为共同的目标。然而，背内侧和背外侧肾小球领域的差异投射到腹侧端脑，前者投射到腹外侧supracommissural区域。逆行标记从腹外侧supracommissural区域显示二尖瓣细胞与2个大肾小球在延髓背内侧区域，puperminal接收输入的隐窝细胞，表明存在一个隐窝细胞嗅觉子系统与单独的预测，在斑马鱼。比较意义的斑马鱼的次级嗅觉通路，传达信息的隐窝细胞进行了讨论。
The olfactory mucosa of the zebrafish consists of 3 morphological types of olfactory receptor neurons (ORNs): ciliated, microvillous, and crypt cells. Previous studies in the zebrafish have revealed differential projections of ciliated and microvillous ORNs, which project to different glomerular fields. However, the bulbar targets of zebrafish crypt cells were not identified. Here, we analyze the relationship between crypt cells of the olfactory epithelium and dorsal glomerular fields of the zebrafish olfactory bulbs, as wells as the connections between these bulbar regions and forebrain regions. For this purpose, a lipophilic carbocyanine tracer (DiI) was used in fixed tissue. Application of DiI to the dorsomedial glomerular field mainly labeled crypt cells in the zebrafish olfactory epithelium. By contrast, application of DiI to the dorsolateral glomerular fields mainly labeled bipolar ORNs and only occasionally crypt cells. Bulbar efferent cells (mitral cells) contacting these dorsal glomerular fields project to different telencephalic areas, with the posterior zone of the dorsal telencephalic area (Dp) as the common target. However, dorsomedial and dorsolateral glomerular fields projected differentially to the ventral telencephalon, the former projecting to the ventrolateral supracommissural region. Retrograde labeling from the ventrolateral supracommissural region revealed mitral cells associated with 2 large glomeruli in the bulbar dorsomedial region, which putatively receives inputs from the crypt cells, indicating the existence of a crypt cell olfactory subsystem with separate projections, in the zebrafish. The comparative significance of the secondary olfactory pathways of zebrafish that convey information from crypt cells is discussed.