Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America.
Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America.
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日本、欧洲和北美伯氏疏螺旋体 OspA 血清型的比较。
DOI：
10.1111/j.1348-0421.1996.tb01106.x
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发表时间：
1996
影响因子：
2.6
通讯作者：
Yanagihara,Y
中科院分区：
文献类型：
--
作者：
Masuzawa,T;Wilske,B;Komikado,T;Suzuki,H;Kawabata,H;Sato,N;Muramatsu,K;Sato,N;Isogai,E;Isogai,H;Johnson,RC;Yanagihara,Y
根据16株抗伯氏疏螺旋体外表面蛋白A(OspA)的单抗和DNA-DNA杂交试验，对来自日本、人和野生动物等不同来源的61株伯氏疏螺旋体和来自俄罗斯远东地区的2株硬蜱进行了分类。在日本和远东地区的俄罗斯分离株中发现了11个OspA血清型(J1到J11血清型鉴定为加里尼氏杆菌，J10血清型鉴定为B.afzelii，J11血清型对应于日本B.jonica)，而北美和欧洲分离株中有7个OspA血清型(Bettina Wilske等，J.Clin)。微生物。31：340-350,1993)，除OspA血清2型与OspA血清J10具有相同的反应性外，其余均未观察到。这一发现为了解莱姆病疏螺旋体的地理分布以及疫苗和诊断试验的发展提供了有用的信息。结论：1.伯氏杆菌在日本尚未发现；2.日本的B.afzelii与欧洲的B.afzelii有较近的亲缘关系；3.日本的B.garinii分离株具有高度的异质性，与欧洲的B.garinii分离株明显不同。
Sixty-one Borrelia burgdorferi sensu lato strains from various sources (ticks, human, and wild animals) in Japan and two strains from ticks in Far Eastern Russia were classified on the basis of reactivity with 16 monoclonal antibodies (mAb) to outer surface protein A (OspA) and by DNA-DNA hybridization assay. Eleven OspA serotypes (J1 to J11) were recognized among the Japanese and the Far East Russian isolates (serotypes J1 to J9 were identified as B. garinii, serotype J10 was identified as B. afzelii, and serotype J11 corresponded to B. japonica), whereas 7 OspA serotypes for North American and European isolates previously reported (Bettina Wilske et al, J. Clin. Microbiol. 31: 340-350, 1993) were not observed except for OspA serotype 2 which showed identical reactivity with OspA serotype J10. This finding provides helpful information for understanding the geographical distribution of Lyme disease borrelia and the development of vaccine and diagnostic tests. In conclusion: 1. B. burgdorferi sensu stricto has not been observed in Japan, 2. Japanese B. afzelii isolates are closely related to those from Europe, 3. B. garinii isolates from Japan are highly heterogeneous and apparently different from European B. garinii isolates.