Characterization of the hepatic DNA damage caused by 1,2-dibromoethane using the alkaline elution technique.
Characterization of the hepatic DNA damage caused by 1,2-dibromoethane using the alkaline elution technique.
复制标题
使用碱性洗脱技术表征 1,2-二溴乙烷引起的肝 DNA 损伤。
DOI：
10.1093/carcin/2.9.839
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发表时间：
1981
期刊：
影响因子：
4.7
通讯作者：
Bowden,GT
中科院分区：
文献类型：
--
作者：
White,RD;Sipes,IG;Gandolfi,AJ;Bowden,GT
腹腔注射对肝细胞 DNA 造成的损伤在雄性瑞士韦伯斯特小鼠中研究了 1,2-二溴乙烷 (EDB) 的给药。治疗后三小时，分离肝细胞核并通过碱性洗脱技术评估 DNA 损伤。分离细胞核的方法保留了 DNA 的完整性，此外还发现纯化的细胞核可以冷冻至少 1 周，而没有检测到 DNA 损伤。 EDB 给药（25-75 mg/kg）导致 DNA 单链断裂呈剂量依赖性增加。当分析前将裂解的细胞核在碱性洗脱溶液中预孵育时，会检测到更多的 DNA 单链断裂。这些碱不稳定位点的存在表明 DNA 链断裂部分是由 EDB 烷基化的 DNA 位点的不稳定造成的。没有证据表明 EDB 诱导 DNA-DNA 交联或 DNA-蛋白质交联。使用分离的肝细胞核作为碱性洗脱分析的样品可能是研究致癌物在体内诱导的 DNA 损伤性质的有用技术。
The damage to hepatic cell DNA caused by i.p. administration of 1,2-dibromoethane (EDB) was studied in male Swiss Webster mice. Three hours after treatment, hepatic nuclei were isolated and damage to DNA assessed by the alkaline elution technique. The method for isolation of the nuclei preserved the integrity of the DNA and in addition it was found that the purified nuclei could be frozen for at least 1 week with no detectable damage to the DNA. EDB administration (25–75 mg/kg) resulted in a dose-dependent increase in DNA single-strand breaks. More DNA single-strand breaks were detected when lysed nuclei were preincubated in the alkaline eluting solution prior to analysis. The presence of these alkali-labile sites suggests that the DNA strand breaks result, in part, from the lability of DNA sites alkylated by EDB. There was no evidence of EDB induced DNA–DNA cross-links or DNA–protein cross-links. The use of isolated hepatic nuclei as a sample for alkaline elution analysis may be a useful technique for studying the nature of DNA damage inducedin vivoby carcinogens.