Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins.
Extravillous trophoblasts invade more than uterine arteries: evidence for the invasion of uterine veins.
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DOI：
10.1007/s00418-016-1509-5
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发表时间：
2017-03
影响因子：
2.3
通讯作者：
Huppertz B
中科院分区：
文献类型：
--
作者：
Moser G;Weiss G;Sundl M;Gauster M;Siwetz M;Lang-Olip I;Huppertz B
在怀孕的前三个月，卵外滋养细胞（evt）侵入子宫肌层的前三分之一的蜕膜间质，从而将胎盘固定在子宫上。它们也沿着血管内和腺体内的途径侵入；堵塞、连接和重塑螺旋动脉，从而在妊娠中期开始时负责血液营养的建立，并在妊娠早期侵入并打开子宫腺体，向绒毛间隙方向进行组织营养营养。本研究的目的是证明子宫静脉在妊娠早期受到类似子宫动脉和子宫腺体的evt侵袭。因此，对孕早期胎盘的连续切片进行免疫单染色和免疫双染色，首先区分动脉和静脉，其次区分浸润血管和非浸润血管。随后，量化evt对子宫血管的侵袭情况。我们的数据显示，在妊娠早期，evt对子宫静脉的侵犯明显多于子宫动脉（29.2±15.7%）。血管横截面显示EVT侵入静脉（59.5%±7.9%）明显高于动脉（29.2±15.7%）。在静脉管腔内，除了分离的腺上皮细胞或合胞体碎片外，还反复发现单个evt。这项研究扩大了我们迄今为止关于妊娠早期EVT侵袭的假设概念。我们认为，在妊娠的前三个月，evt侵入子宫静脉是导致废物和血浆从绒毛间隙排出的原因。
During the first trimester of pregnancy, extravillous trophoblasts (EVTs) invade into the decidual interstitium to the first third of the myometrium, thereby anchoring the placenta to the uterus. They also follow the endovascular and endoglandular route of invasion; plug, line and remodel spiral arteries, thus being responsible for the establishment of hemotrophic nutrition with the beginning of the second trimester and invade and open uterine glands toward the intervillous space for a histiotrophic nutrition during the first trimester. The aim of this study was to provide proof that uterine veins are invaded by EVTs similar to uterine arteries and glands in first trimester of pregnancy. Therefore, serial sections from in situ first trimester placenta were immuno-single- and immuno-double-stained to distinguish in a first step between arteries and veins and secondly between invaded and non-invaded vessels. Subsequently, invasion of EVTs into uterine vessels was quantified. Our data show that uterine veins are significantly more invaded by EVTs than uterine arteries (29.2 ± 15.7 %) during early pregnancy. Counted vessel cross sections revealed significantly higher EVT invasion into veins (59.5 ± 7.9 %) compared to arteries (29.2 ± 15.7 %). In the lumen of veins, single EVTs were repeatedly found, beside detached glandular epithelial cells or syncytial fragments. This study allows the expansion of our hitherto postulated concept of EVT invasion during first trimester of pregnancy. We suggest that invasion of EVTs into uterine veins is responsible the draining of waste and blood plasma from the intervillous space during the first trimester of pregnancy.