Novel Chlamydia pneumoniae vaccine candidates confirmed by Th1-enhanced genetic immunization.
Novel Chlamydia pneumoniae vaccine candidates confirmed by Th1-enhanced genetic immunization.
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Th1 增强基因免疫证实了新型肺炎衣原体候选疫苗。
DOI：
10.1016/j.vaccine.2009.11.046
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发表时间：
2010
期刊：
影响因子：
5.5
通讯作者：
Kaltenboeck,Bernhard
中科院分区：
文献类型：
--
作者：
Li,Yihang;Ahluwalia,SudhirK;Borovkov,Alexandre;Loskutov,Andrey;Wang,Chengming;Gao,Dongya;Poudel,Anil;Sykes,KathrynF;Kaltenboeck,Bernhard
高免疫原性抗原的鉴定对于构建有效的肺炎衣原体亚单位疫苗至关重要。之前的一个项目使用全基因组筛选来识别12种保护性C。在A/J小鼠肺疾病模型中检测肺炎链球菌候选基因（Li等人[14]）。由于Th 1免疫的诱导不足，这些基因仅引起适度的保护。在这里，我们使用大肠杆菌不耐热肠毒素作为Th 1增强基因佐剂，并重新测试这12个基因，与其他研究人员确定的6个基因平行。疫苗候选基因cutE和Cpn 0420通过所有评估的标准赋予显著的保护（预防C.肺炎引起的死亡，减少肺部疾病，消除C.肺炎）。oppA_2基因在病害减轻和C.肺炎消除。其他四个基因在一个标准下具有保护作用。其他地方报道的六个基因中没有一个能通过减少肺部疾病或消除C.肺炎，但三人通过增加生存率而受到保护。
Identification of highly immunogenic antigens is critical for the construction of an efficacious subunit vaccine against Chlamydia pneumoniae infections. A previous project used a genome-wide screen to identify 12 protective C. pneumoniae candidate genes in an A/J mouse lung disease model (Li et al. [14]). Due to insufficient induction of Th1 immunity, these genes elicited only modest protection. Here, we used the Escherichia coli heat-labile enterotoxin as a Th1-enhancing genetic adjuvant, and re-tested these 12 genes, in parallel with six genes identified by other investigators. Vaccine candidate genes cutE and Cpn0420 conferred significant protection by all criteria evaluated (prevention of C. pneumoniae-induced death, reduction of lung disease, elimination of C. pneumoniae). Gene oppA_2 was protective by disease reduction and C. pneumoniae elimination. Four other genes were protective by a single criterion. None of the six genes reported elsewhere protected by reduction of lung disease or elimination of C. pneumoniae, but three protected by increasing survival.