Developmental Ability of Trophoblast Stem Cells in Uniparental Mouse Embryos
Developmental Ability of Trophoblast Stem Cells in Uniparental Mouse Embryos
复制标题
DOI：
10.1016/j.placenta.2009.02.006
复制
发表时间：
2009-05-01
期刊：
影响因子：
3.8
通讯作者：
Kono, T.
中科院分区：
文献类型：
--
作者：
Ogawa, H.;Shindo, N.;Kono, T.
由于包括胎盘在内的胚胎外组织发育不足，单性生殖（PG）和雄激素生殖（AG）小鼠胚胎在妊娠第9.5天后都不能存活。妊娠第9.5天，PC胚胎胎盘结构发育不全，而AG胚胎着床部位滋养细胞巨细胞丰富。这些发现表明，双亲的基因组都是胎盘发育所必需的。为了进一步了解PC和AC胚胎的滋养细胞谱系，我们试图从单亲代胚胎中获得滋养细胞干（TS）样细胞系。此外，我们试图通过基因表达分析来评估它们向滋养细胞谱系分化的能力。从AG胚胎中获得了表达未分化TS细胞标记基因（Cdx2和Err β）的三个细胞系。在分化条件下，这些细胞表达滋养层巨细胞特异性基因，但不表达海绵状滋养层特异性基因。相比之下，来自PC胚胎的四种细胞系都没有表达未分化TS细胞的标记基因，但它们表达了胚胎干细胞的标记基因Oct3/4。免疫组化分析显示，PC囊胚内细胞团和滋养外胚层分别特异表达Oct3/4和Cdx2。这些结果表明，由于滋养细胞的发育能力不足，PC胚胎不具有TS细胞。2009爱思唯尔有限公司版权所有。
Neither parthenogenetic (PG) nor androgenetic (AG) mouse embryos survive after day 9.5 of pregnancy, owing to the inadequate growth of extraembryonic tissues, including the placenta. At day 9.5 of pregnancy, the placental structures are poorly developed in PC embryos, while trophoblast giant cells are abundant at the implantation site in AG embryos. These findings suggest that both parental genomes are required for placental development. To gain further insight into the trophoblast lineage in PC and AC embryos, we attempted to derive trophoblast stem (TS)-like cell lines from uniparental embryos. Furthermore, we sought to assess their ability to differentiate into cells of the trophoblast lineage by using gene expression analysis. Three cell lines that expressed marker genes for undifferentiated TS cells (Cdx2 and Err beta) were derived from AG embryos. Under differentiation conditions, these cells expressed the trophoblast giant cell-specific genes, but did not express the spongiotrophoblast-specific genes. In contrast, none of the four cell lines from PC embryos expressed marker genes for undifferentiated TS cells, but they expressed Oct3/4, a marker gene for embryonic stem cells. Immunohistochemical analysis indicated that PC blastocysts expressed Oct3/4 and Cdx2 specifically in inner cell mass and the trophectoderm respectively. These results suggest that PC embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells. (C) 2009 Elsevier Ltd. All rights reserved.