mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p
mcm5/cdc46-bob1 bypasses the requirement for the S phase activator Cdc7p
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DOI：
10.1073/pnas.94.7.3151
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发表时间：
1997-04-01
影响因子：
11.1
通讯作者：
Sclafani, RA
中科院分区：
文献类型：
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作者：
Hardy, CFJ;Dryga, O;Sclafani, RA
Cdc 7 p是酿酒酵母中G（1）/S转换和DNA复制起始所需的蛋白激酶。Cdc 7 p及其底物发挥作用的机制尚不清楚。在酿酒酵母中，MCM家族的一个成员MCM 5/CDC 46中存在隐性突变，该突变绕过了对Cdc 7 p及其相互作用因子Dbf 4p的需要。由于进化上保守的MCM家族蛋白参与了限制DNA复制到每个细胞周期一次的过程，我们的研究表明Cdc 7 p是G（1）晚期所必需的，因为在它缺失的情况下，Mcm 5 p/Cdc 46 p阻断了DNA复制的起始。此外，Mcm 5 p/Cdc 46 p可能对细胞启动复制的能力具有积极和消极的影响。
Cdc7p is a protein kinase that is required for G(1)/S transition and initiation of DNA replication in Saccharomyces cerevisiae. The mechanisms whereby Cdc7p and its substrates exerts their effects are unknown, We report here the characterization in S. cerevisiae of a recessive mutation in a member of the MCM family, MCM5/CDC46, which bypasses the requirement for Cdc7p and its interacting factor Dbf4p. Because the MCM family of evolutionarily conserved proteins have been implicated in restricting DNA replication to once per cell cycle, our studies suggest that Cdc7p is required late In G(1) because in its absence the Mcm5p/Cdc46p blocks the initiation of DNA replication. Moreover, Mcm5p/Cdc46p may have both positive and negative effects on the ability of cell to initiate replication.