Airborne Fungi Induce Nasal Polyp Epithelial Cell Activation and Toll-Like Receptor Expression
Airborne Fungi Induce Nasal Polyp Epithelial Cell Activation and Toll-Like Receptor Expression
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DOI：
10.1159/000301578
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Lee, Young-Ho
中科院分区：
文献类型：
--
作者：
Shin, Seung-Heon;Lee, Young-Ho
背景：鼻上皮是空气中变应原遇到的第一道屏障，是气道炎症的积极参与者。真菌越来越被认为是鼻窦炎和气道疾病的重要病原体。本研究的目的是评估鼻息肉上皮细胞中细胞因子产生过程中真菌蛋白酶的活性，并确定真菌Toll样受体（TLR）mRNA的表达。方法：从患者鼻息肉中分离鼻息肉上皮细胞，用链格孢菌和曲霉菌进行刺激。测定白细胞介素-8（IL-8）和粒细胞巨噬细胞集落刺激因子（GM-CSF）以确定上皮细胞的活化。逆转录聚合酶链反应检测鼻黏膜上皮细胞TLR mRNA的表达。用蛋白酶抑制剂和抗人TLR抗体抑制细胞因子的产生。结果：真菌可促进鼻黏膜上皮细胞产生IL-8和GM-CSF。当鼻上皮细胞被真菌激活时，TLR2、TLR3和TLR4 mRNA的表达比未激活的细胞更强。细胞因子的产生被蛋白酶抑制剂和抗人TLR4抗体抑制。结论：本研究结果表明，真菌与鼻上皮细胞相互作用，促进细胞因子的产生和TLR mRNA的表达。细胞因子的产生与真菌中的蛋白酶和TLR4有关。版权所有（C）2010 S. Karger AG，巴塞尔
Background: The nasal epithelium is the first barrier encountered by airborne allergens and is an active participant in airway inflammation. Fungi have been increasingly recognized as important pathogens in sinusitis and airway diseases. The aim of the study was to evaluate fungal protease activity during cytokine production in nasal polyp epithelial cells and to determine the expression of Toll-like receptor (TLR) mRNA by fungi. Methods: Nasal polyp epithelial cells were obtained from patients and stimulated with Alternaria and Aspergillus. Interleukin-8 (IL-8) and granulocyte macrophage colony-stimulating factor (GM-CSF) were measured to determine the activation of epithelial cells. Reverse transcriptase polymerase chain reaction for the TLR mRNA expression of the nasal epithelial cells was performed. Cytokine production was inhibited with protease inhibitors and anti-human TLR antibodies. Results: The fungi enhanced the production of IL-8 and GM-CSF from nasal epithelial cells. When nasal epithelial cells were activated by the fungi, TLR2, TLR3 and TLR4 mRNAs were more strongly expressed than in the nonactivated cells. Cytokine production was inhibited by protease inhibitors and anti-human TLR4 antibodies. Conclusions: The results of this study showed that fungi interacted with nasal epithelial cells and enhanced the production of cytokines and TLR mRNA expression. The cytokine production was related to the protease in fungi and TLR4. Copyright (C) 2010 S. Karger AG, Basel