The effect of conjugated linoleic acid on transepithelial calcium transport and mediators of paracellular permeability in human intestinal-like Caco-2 cells
The effect of conjugated linoleic acid on transepithelial calcium transport and mediators of paracellular permeability in human intestinal-like Caco-2 cells
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DOI：
10.1016/j.plefa.2004.10.015
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发表时间：
2005-03-01
影响因子：
3
通讯作者：
Cashman, KD
中科院分区：
文献类型：
--
作者：
Jewell, C;Cusack, S;Cashman, KD
共轭亚油酸(CLA)增加了人肠样Caco-2细胞单层的细胞旁通透性，该单层主要通过跨细胞途径转运钙。然而，在体内，细胞外钙转运是钙转运的主要途径。因此，本研究的目的是探讨共轭亚油酸对主要通过细胞旁途径转运钙的Caco-2细胞跨皮钙转运的影响。将细胞接种到可渗透的运输膜上，并允许其在14天内分化为肠样细胞单层。在钙转运研究之前，单层细胞(n=9/处理)暴露于0(对照)或80um-18：2，-cis-9，反式-11共轭亚油酸或-反式-10，顺式-12共轭亚油酸14d。总的跨上皮钙转运以及跨细胞和近细胞钙转运显著增加(P
Conjugated linoleic acid (CLA) increases paracellular permeability across human intestinal-like Caco-2 cell monolayers, which transport Ca predominantly by the transcellular route. In vivo, however, paracellular Ca transport is the predominant route of Ca transport. Therefore, the objective of this study was to investigate the effect of CLA on transepithelial Ca transport in Caco-2 cells transporting Ca predominantly by the paracellular route. Cells were seeded onto permeable transport membranes and allowed to differentiate, over 14 d, into intestinal-like cell monolayers. Monolayers (n = 9/treatment) were exposed to 0 (control) or 80 muM-18:2, -cis-9, trans-11 CLA or -trans-10, cis-12 CLA for 14d prior to Ca transport studies. Overall transepithelial Ca transport as well as transcellular and parcellular Ca transport was significantly increased (P