PROTEIN SEQUENCING BY TANDEM MASS-SPECTROMETRY
PROTEIN SEQUENCING BY TANDEM MASS-SPECTROMETRY
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DOI：
10.1073/pnas.83.17.6233
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发表时间：
1986-09-01
影响因子：
11.1
通讯作者：
HAUER, CR
中科院分区：
文献类型：
--
作者：
HUNT, DF;YATES, JR;HAUER, CR
描述了用串联质谱法测定蛋白质氨基酸序列的方法。该方法包括酶和/或化学降解蛋白质到肽的集合，然后通过高效液相色谱分离。每个片段含有多达10-15个肽段，然后在多分析仪仪器上通过液体二次离子/碰撞激活解离质谱法的组合直接分析，而无需进一步纯化。描述了碰撞激活解离质谱的解释，并给出了用溴化氰和胰蛋白酶处理载脂蛋白B产生的可溶性肽的研究结果。
Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.