Correlation of early auditory potentials and intracochlear electrode insertion properties: an animal model featuring near real-time monitoring.
Correlation of early auditory potentials and intracochlear electrode insertion properties: an animal model featuring near real-time monitoring.
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DOI：
10.1097/mao.0b013e3181f6c899
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发表时间：
2010-12
期刊：
影响因子：
--
通讯作者：
Adunka OF
中科院分区：
文献类型：
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作者：
Campbell AP;Suberman TA;Buchman CA;Fitzpatrick DC;Adunka OF
本研究的目的是在听力保留的人工耳蜗植入动物模型中，评估耳蜗腔内电极撞击耳蜗结构时对声刺激的电生理反应的变化。最终目标是开发有效的程序来评估手术中使用的耳蜗生理学状态。对16只沙土鼠和18只耳朵进行了测试。经耳蜗底转造口置入刚性电极，指向基底膜/骨性螺旋板复合体。随着电极的推进，记录包括耳蜗微音学(CMS)和复合动作电位(CAPS)在内的听诱发早期听觉电位到一个由一个刺激频率和强度组成的短刺激序列。使用显微内窥镜观察电极插入过程并确定电极撞击的位置。每次实验结束后，采用整装准备方法确定损伤部位。电生理改变与损伤程度和部位密切相关。我们观察到了四种不同的模式。此外，内窥镜结合短记录序列允许检测当电极收回时可逆的反应变化。这些病例与组织学上的全层损伤有关。通过短的记录序列获得听觉诱发的内耳电位和显微内窥镜，我们可以检测到不同程度的耳蜗伤，包括轻微和可逆的损伤。使用目前的植入技术，这种类型的录音可能是可用的。未来在测量方面的改进有望提高记录范例的效率，以产生临床上有用的工具。
The goal of this work was to assess electrophysiologic response changes to acoustic stimuli as an intracochlear electrode impacted cochlear structures in an animal model of hearing preservation cochlear implantation. The ultimate goal is to develop efficient procedures for assessing the status of cochlear physiology for intraoperative use. Sixteen gerbils and 18 ears were tested. A rigid electrode was inserted through a basal turn cochleostomy and directed towards the basilar membrane/osseous spiral lamina complex. We recorded acoustically evoked early auditory potentials including cochlear microphonics (CMs) and compound action potentials (CAPs) to a short stimulation sequence consisting of one stimulus frequency and intensity as the electrode was advanced. A microendoscope was used to visualize the electrode insertion progress and to identify the site of electrode impact. After each experiment, the site of intracochlear trauma was confirmed using whole mount preparations. Electrophysiologic changes correlated well with the degree and location of trauma. We observed four distinct patterns. In addition, the endoscope in conjunction with the short recording sequence allowed for the detection of response changes that were reversible when the electrode was retracted. These cases were associated with less than full-thickness damage on histology. The short recording sequence to obtain acoustically evoked intracochlear potentials and the microendoscope allowed us to detect various levels of cochlear trauma including minor and reversible damage. Recordings of this type are potentially available using current implant technology. Future improvements in the measurements can be expected to improve the efficiency of the recording paradigm to produce a clinically useful tool.