Enantioselective Artificial Metalloenzymes by Creation of a Novel Active Site at the Protein Dimer Interface
Enantioselective Artificial Metalloenzymes by Creation of a Novel Active Site at the Protein Dimer Interface
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DOI：
10.1002/anie.201202070
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发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Roelfes, Gerard
中科院分区：
文献类型：
--
作者：
Bos, Jeffrey;Fusetti, Fabrizia;Roelfes, Gerard
人工金属酶是通过在转录因子LmrR的二聚体界面上形成新的活性位点而产生的。两个铜中心通过与二聚体的每一半中的配体结合而并入。用这个系统高达97%ee，在基准铜II催化的Diels-Alder反应中获得。
Artificial metalloenzymes are generated by forming a novel active site on the dimer interface of the transcription factor LmrR. Two copper centers are incorporated by binding to ligands in each half of the dimer. With this system up to 97% ee was obtained in the benchmark CuII catalyzed Diels–Alder reaction.