Effects of cysteine in serum-free maturation medium on male pronuclear formation of maturing pig oocytes penetrated in vitro
Effects of cysteine in serum-free maturation medium on male pronuclear formation of maturing pig oocytes penetrated in vitro
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DOI：
10.1262/jrd.43.73
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发表时间：
1997-02
影响因子：
1.8
通讯作者：
K. Sawai;H. Funahashi;Wei‐Hua Wang;K. Niwa
中科院分区：
文献类型：
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作者：
K. Sawai;H. Funahashi;Wei‐Hua Wang;K. Niwa
。将猪未成熟卵母细胞在含或不含 0.57 mM 半胱氨酸的无血清成熟培养基中培养 24、36 和 48 小时。培养基中添加半胱氨酸与卵母细胞谷胱甘肽合成增加有关，但不会促进或抑制核成熟、精子体外穿透以及穿透卵母细胞中精子核的解凝。在体外受精后 14 小时，在半胱氨酸存在和不存在的情况下培养 24 小时的穿透卵母细胞的激活率低于 36 小时和 48 小时。无论是在受精培养基中添加半胱氨酸（0.57 mM），还是延长授精后的培养时间，都没有改善培养24小时的卵母细胞被激活的较低能力。然而，当在成熟培养基中添加半胱氨酸时，在成熟的任何阶段精子穿透后激活的卵母细胞中雄性原核的形成都大大加速。谷胱甘肽浓度的增加可能会诱导体外穿透的未成熟猪卵母细胞中精子核的完全去浓缩，确保仅与卵母细胞激活同步地将去浓缩的精子核转化为雄性原核。重现。 73–80,
. Pig immature oocytes were cultured for 24, 36 and 48 h in serum-free maturation medium with or without 0.57 mM cysteine. The addition of cysteine to the medium was associated with increased glutathione synthesis by oocytes but did not promote nor inhibit nuclear maturation, sperm penetration in vitro , and decondensation of sperm nuclei in penetrated oocytes. The incidence of activation of penetrated oocytes 14 h after insemination in vitro was lower in those cultured both in the presence and absence of cysteine for 24 h than 36 and 48 h. The lower ability of oocytes cultured for 24 h to be activated was not improved by neither the addition of cysteine (0.57 mM) in fertilization medium nor prolonged culture time after insemination. However, male pronuclear formation in activated oocytes after sperm penetration at any stages of maturation was largely accelerated when cysteine was added to maturation medium. An increased concentration of glutathione may induce full decondensation of sperm nuclei in immature pig oocytes penetrated in vitro ensuring transformation of the decondensed sperm nuclei to male pronuclei only in synchronization with oocyte activation. Reprod. 73–80,