Selective lung leukosequestration after complement activation.
Selective lung leukosequestration after complement activation.
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补体激活后选择性肺白细胞隔离。
DOI：
10.1152/jappl.1988.65.1.80
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Hechtman,HB
中科院分区：
文献类型：
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作者：
Klausner,JM;Kobzik,L;Valeri,CR;Shepro,D;Hechtman,HB
这项研究测试了激活的补体是否会导致肺中多形核白细胞(PMN)的选择性捕获。用酵母多糖激活血浆(ZAP，2.5 mg/ml)按5ml/min的速度注入清醒绵羊上腔静脉(IV，n=4)或主动脉弓或股动脉(IA，n=8)。静脉输注结束时，白细胞数由8862个/mm3降至1631个/mm3(P<0.01)。全肺PMN计数下降了74%。血浆血栓素B2由114 pg/ml升至2,733 pg/ml(P<0.01)，平均肺动脉压由12升至42 mm Hg(P<0.01)，生理分流由13%升至25%(P<0.05)。1h内淋巴液TxB2水平从301上升到4916pg/ml(P<0.01)，肺淋巴流量(QL)从3.7上升到11.1ml/30min(P<0.05)，淋巴/血浆蛋白比值(L/P)保持在0.63不变，淋巴蛋白清除率从2.3上升到7.5ml/30min(P<0.05)。肺、脾相对假手术组(P<0.05)，其他脏器未见白血球，用毛细血管PMN计数和测定粒细胞标志物髓过氧化物酶定量。动脉内注入ZAP导致的变化在幅度和时间上与静脉注射组相似。
This study tests whether activated complement leads to a selective entrapment of polymorphonuclear leukocytes (PMN's) in the lungs. Awake sheep were infused for 5 min with zymosan-activated plasma (ZAP, 2.5 mg/ml) at a rate of 5 ml/min into the superior vena cava (IV, n = 4) or intra-arterially into the aortic arch or femoral artery (IA, n = 8). At the end of IV infusion, leukocyte counts fell from 8,862 to 1,631/mm3 (P less than 0.01). PMN counts across the lungs decreased by 74%. There were increases in plasma thromboxane (Tx) B2 from 114 to 2,733 pg/ml (P less than 0.01), mean pulmonary arterial pressure from 12 to 42 mmHg (P less than 0.01), and physiological shunt from 13 to 25% (P less than 0.05). Within 1 h lymph TxB2 levels had risen from 301 to 4,916 pg/ml (P less than 0.01), lung lymph flow (QL) rose from 3.7 to 11.1 ml/30 min (P less than 0.05), lymph-to-plasma protein ratio (L/P) remained unchanged at 0.63, and lymph protein clearance increased from 2.3 to 7.5 ml/30 min (P less than 0.05). Leukosequestration, quantitated by capillary PMN counting and by assaying the granulocyte marker myeloperoxidase, occurred relative to sham animals (P less than 0.05) in the lung and spleen but not in other organs. Intra-arterial ZAP infusion led to changes that were similar in magnitude and timing to the IV group.(ABSTRACT TRUNCATED AT 250 WORDS)